Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, with acceptable restrictions (e.g. non-GLP, no analytical verification of test item concentrations).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- test substance: N-Methylacetamide
- purity: > 99 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- species: Pseudomonas putida, DSM 50026
- incubated at 24 °C (297 K +- 1 K), 150 rpm for 7+-1 h
- medium: AK-medium according to DIN 38412, Part 8 (draft)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Details on test conditions:
- test vessel: 300 ml-Erlenmeyer flasks, 1 baffle
- liquid volume: 100 ml
- liquid volume: 10 ml (0.5 ml nutrient solution concentrate I (20x), 0.5 ml nutrient solution concentrate II (20x), 1 ml inoculum, 8 ml test item solution) - inoculum: 1 ml pre-culture (adjusted to standardized turbidity)
- test concentrations (mg/l): 0 (control), 312.5, 625, 1250, 2500, 5000, and 10000
- replicates: inoculated: 4 per concentration and control
- incubated at 20 °C (293 K)
- measurements: photometric determination at 436 nm
- pH determined at test start (0 h) and after 17 h
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
17 h
Dose descriptor:
other: TTC (Toxicity threshold concentration, comparable to EC10)
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

RS-Freetext:
Concentration (mg/l) Growth (% of control)
0 (control) 100
312.5 90.74
625 90.84
1250 103.42
2500 103.63
5000 102.03
10000


pH at test start and test end:

test conc. uninoculated uninoculated
(mg/l) (0 h) (17 h)
0 (control) 7.2 4.0
312.5 7.2 4.4
625 7.2 4.5
1250 7.2 5.0
2500 7.2 4.7
5000 7.2 4.0
10000 7.1 3.6

Applicant's summary and conclusion