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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (e.g. no analytical verification of test concentrations)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German Industrial Standard DIN 38412, part 11
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-methylacetamide
EC Number:
201-182-6
EC Name:
N-methylacetamide
Cas Number:
79-16-3
Molecular formula:
C3H7NO
IUPAC Name:
N-methylacetamide
Details on test material:
- test substance: N-Methylacetamide
- purity: no data

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: German Federal Health Agency, Berlin
- breeding method: single breeding
- 1 animal in 100 ml beaker with 70 ml medium
- reproduction rate: 3 animals/d

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Details on test conditions:
- test medium: according to German Industrial Standard DIN 38412, part 11
- temperature: 21 °C
- test vessels: 50-ml glass beaker (DIN 12331)
- test volume: 20 ml (4 ml/animal)
- 5 animals per vessel
- 20 animals per concentration level (= 4 replicates)
- illumination: diffuse light (10 µE/m²*s)
- observation times: visually after 0, 3, 6, 24 and 48 h
- observation parameters: mobility
- test concentrations: 0 (control), 58, 100, 180, 320, and 580 mg/l
- reference substance: potassium dichromate
- EC 50 after 24 h and EC 50 after 48 h were determined according to Spearman-Kärber (Sachs, L. (1984): Angewandte Statistik, Springer, Berlin.)
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

RS-Freetext:
Summary of test results
Results after
3h 6h 24h 48h
EC0 (mg/l) >= 580 >= 580 >= 580 >= 580
EC50 (mg/l) > 580 > 580 > 580 > 580
EC100 (mg/l) > 580 > 580 > 580 > 580


The results were obtained at 21 °C, while the test
medium had the following specifications:
oxygen 8.0 mg/l
pH 7.42-7.67


Test results:

Immobility:
Nominal Cumulative immobility (n)
Concentration (mg/l) 0 h 3 h 6 h 24 h 48 h
0 (Control) 0 0 0 0 0
100 0 0 0 0 0
180 0 0 1 1 1
320 0 0 0 0 0
580 0 0 1 1 1

Reference substance:
EC0: 1.0 mg/l
EC50: 1.45 mg/l
EC100: 3.2 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes