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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: According to guideline , undissolved test substance was observed

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
other: DIN 38412 Testverfahren mit Wasserorganismen (L15)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
No 87/172

Test solutions

Vehicle:
no
Details on test solutions:

test water: reconstituted freshwater according to DIN 38 412 part 11, draft 1981. Preparation from fully demineralised tap water, conductivity: max 10 micro MHO
PREPARATION OF THE TEST SUBSTANCE : THE PRODUCT WAS ADDED TO THE TEST WATER WITHOUT ANY PRETREATMENT ; SUBSEQUENTLY THE FISH WERE PLACED INTO THE AQUARIA .

Acid capacity: ca. 5.5mmol/L
Test water
reconstitueted freshwater according to DIN 38 412, part 11, Draft Sept.1981, preparation from fully demineralised tap water (Conductivity: Max 10 micro MHO
Resalting:
294 mg/L CaCl2 3 2H2O
123.3 mg/L MGSO4 3 7H2O
64.8mg/l NaHCO3
5.8 mg/L KCl
aeration with oil-free air

Test water ready for use:
total hardness: 2.5 mmol/L
Acid capacit: 0.8 mmol/L
ratio Ca/Mg Ions: 4:1
Ratio Na/K ions: 10:1
pH: ca. 7.8

volume of test water: 10l

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
Breeder/supplier: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
body length: 5.7 cm
body weight: 2.8g
Corpulence factor of the batch (K, is calculated from weight W8g) and length L (cm) measured from the tip of the mouth to the end of thetailfin according to the formula K=100xW/Lxx3

Housing:
flowthrough tank in tap water
Hardness 2.5mm0l/L, acid capacity 5.5mmol/L, oxygen content >60% of maximum saturation, pH ca. 8.0, 21°C
duration ca 3 weeks
medical treatment: 2x 0.05mg/L malachite green chloride, 1x 10mg/L tetracycline hydrochloride
diet: growing feed F/B 50, SSNIFF Spezialdiaeten GmbH, 4470 Soest, Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5mmol/ L
Test temperature:
21°C
pH:
7.8
Dissolved oxygen:
>60% of maximum saturation
Salinity:
RATIO CA/MG IONS 4:1
RATIO NA/K IONS 10:1
Nominal and measured concentrations:
REASONS FOR THE SELECTION OF THE CONCENTRATIONS :
BASED ON THE RESULTS OF A RANGE FINDING STUD Y (LC 50 AFTER 96 H : BETWEEN 10 AND 100 MG/L)
4 .64, 10 .0, 21 .5, 46 .4 AND 100 MG/L .
Details on test conditions:
TEST SYSTEM
- Test vessel: glass: 30cm x 22cm x 24 cm
- fill volume: 10L
- Aeration: slight, continuously with oil-free air
- No. of organisms per vessel: 10
- Biomass loading rate: 2.8g fish/ L test water
- Photoperiod: 16 hours light, 8 hours darkness
adaption to test water 3 days
withdrawal of food: 1 day
Duration of housing and adaption: ca 3 weeks
Mortality during the last 2 weeks of housing:0%
Mortality during the adaption period: 0%



determination of mortality and symptoms: 1h ,4h, 24h, 48h, 72h, 96h
Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
1 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
4 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 22 - < 46 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 22 - < 46 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 22 - < 46 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 22 - < 46 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
>= 46.4 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Details on results:
undissolved test substance was visible on the bottom of the aquaria
no abnormalities were observed
Results with reference substance (positive control):
LC50 (48h) 24mg/L (Sept 28, 1987), corresponds to the normal sensitivity

Applicant's summary and conclusion