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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed prior to international guidelines. Performed according to GLPs. Used 5 test concentrations and negative/positive controls.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Committee on methods for toxicity tests with aquatic organisms. 1975. Methods for acute toxicity tests with fish, macroinvertebrates adn amphibians. EPA, Ecological Research Series EPA-660/3-75-009, April, 1975. 61 pp.
Principles of method if other than guideline:
Static test. 5 test concentraitons plus negative and positive controls. Observations at 24, 48, 96 hr post addition of test material to tanks.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
FM PHT-4 Diol commercial product.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
See below.

Test organisms

Test organisms (species):
Lepomis macrochirus
Details on test organisms:
See below.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Hardness:
45 mg/L CaCO3
Test temperature:
22 +/- 1 degrees C
pH:
7.0
Dissolved oxygen:
9.4 mg/L
Nominal and measured concentrations:
FM PHT-4 Diol: 0, 10, 18, 32, 56 and 100 mg/L.
Antimycin A: positive control
Reference substance (positive control):
yes
Remarks:
Antimycin A

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: The 95% CI was 0-18 mg/L.
Details on results:
At the 32, 56 and 100 mg/L concentrations, 100% mortality was observed at 24 hr. In the 18 mg/L concentration, 90% and 100% mortality were observed at 24 and 48 hrs, respectively. At the 10 mg/L concentration, no mortality was observed at 24 and 48 hr and 20% mortality was observed at 96 hrs. No mortality occurred in the negative control group.
Results with reference substance (positive control):
Performed as expected. 96 hrLC50=0.000056 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
An acute static fish toxicity study was conducte with FM PHT-4 Diol. Bluegill sunfish were exposed to concentrations of 10, 18, 32, 56 and 100 mg test substance/L water. Acetone was used as vehicle. All test concentrations were cloudy with the 56 and 100 mg/L levels being completely opaque. The 96-hr LC50 was 12 mg/L with 95% confidence limits of 0-18 mg/L. A 96 -hr no observed effect concentration of <10 mg/L was indicated.
Executive summary:

An acute static fish toxicity study was conducte with FM PHT-4 Diol. Bluegill sunfish were exposed to concentrations of 10, 18, 32, 56 and 100 mg test substance/L water. Acetone was used as vehicle. All test concentrations were cloudy with the 56 and 100 mg/L levels being completely opaque. The 96-hr LC50 was 12 mg/L with 95% confidence limits of 0-18 mg/L. A 96 -hr no observed effect concentration of <10 mg/L was indicated.