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EC number: 215-035-9 | CAS number: 1271-19-8
The results of the sieving procedure are shown in the following table:
Mass of test item transferred to sieve
Mass of test item passed through sieve
Percentage of test item less than 100 µm
The results of the cascade impactor procedure are shown in the following table:
Particle size range collected (µm)
Collected mass (g)
5.5 to 10.0
2.4 to 5.5
1.61 to 2.4
0.307 to 1.61
Total mass of collected test item
Mass of test item added
 A minor apparent weight loss of approximately 1 mg was noted for the final filter stage, for this determination only. This anomaly was assessed as having no significant impact on the validity or integrity of the evaluation of particle size distribution for the test item given the extremely limited proportion of test item within the valid quantification range of the cascade impactor. A collected weight of 0.0000 g was used for continued calculations of cumulative masses and mass percentages.
The cumulative amounts of test item collected in the three determinations for the individual particle size cut-points are shown in the following table:
Particle size cut point (µm)
Cumulative mass (g)
Cumulative percentage (%)
Overall cumulative percentage with a particle size less than 10.0 µm: ≤1.52%
Overall cumulative percentage with a particle size less than 5.5 µm : ≤0.41%
Sampling for the cascade impactor determinations was performed by rolling the test item container for approximately 10 minutes then sampling from the top, middle and bottom.
The overall results from the cascade impactor were taken as limit values due to the limited but variable low particle size fractions collected across the multiple sampling positions; this gives a worst case scenario. Too few particles were of a size less than 10.0 µm to allow accurate assessment of the mass median aerodynamic diameter.
Particle size data was acquired, using a procedure designed to be compatible with the European Commission Technical Guidance Document EUR 20268 Determination of Particle Size Distribution, Fibre Length and Diameter Distribution of Chemical Substances (2002). The results are as follows:
Percentage of test item with an inhalable particle size <100 µm
Percentage of test item with a thoracic particle size <10.0 µm
Percentage of test item with a respirable particle size <5.5 µm
Percentage of test item with an inhalable particle size <100 µm, 75% (measured via sieve)
Percentage of test item with a thoracic particle size <10.0 µm, ≤1.52% (measured via cascade impactor)
Percentage of test item with a respirable particle size <5.5 µm, ≤0.41% (measured via cascade impactor)
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