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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
96 hours
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Young hatchery-purchased fry (6.2 + 0.7 cm; 1.7 + 0.7 g), acclimated for at least 3 weeks to the treated city water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Tests were carried out using activated charcoal and ultraviolet light-treated Longueuil-city water, whose hardness generally stands between 100 to 150 mg L -I in CaCO 3 equivalents.
Test temperature:
15 +/- 0.1 deg C
pH:
pH (value not reported) was monitored periodically throughout the experiment.
Dissolved oxygen:
Aeration (7.5 cm 3 L -I min -1) of each test vessel. Dissolved oxygen (value not reported) was monitored periodically throughout the experiment.
Nominal and measured concentrations:
0 (control), 0.01, 0.1, 1, 10 and 100 mg/L Zr.
Details on test conditions:
Exposure vessels consisted of 60 L Rubbermaid containers lined with a polyethylene bag into which was placed a black-meshed Vexar (Dupont) cylinder (with lid) espousing the contour of the assay container.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 20 mg/L
Conc. based on:
element
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 54 mg/L
Nominal / measured:
nominal
Conc. based on:
other: Registered substance
Basis for effect:
mortality (fish)
Details on results:
100% mortality within 2 hr at 100 mg/L Zr (pH-3.0), but identical effect for dilution water adjusted to pH=3.0. In the 96-hr LCs0 bioassay with rainbow trout, low pH was entirely responsible
for toxicity at 100 mg/L Zr. At 10 mg L I Zr (pH = 6.7), the next highest test concentration used in the assay, no lethal effects were observed. The LC50 was therefore initially identified as being > 10 mg L -1 Zr. However, in a subsequent sublethal bioassay carried out at 0, 1, 5 and 20 mg/L Zr, no mortality was noted at the highest concentration tested. Hence, a 96-hr LC50 > 20 mg L -1 was finally reported.
Sublethal observations / clinical signs:

The reported toxicity of zirconium 4+ ion was used to calculate the toxicity of the registered substance based on a 37% Zr w/w content

Conclusions:
The LC50 (96 hr) in rainbow trout (Salmo gairdneri) was reported to be >20 mg/L Zr, calculated to be 54 mg/l for the registered substance.
Executive summary:

The LC50 (96 hr) in rainbow trout (Salmo gairdneri) was reported to be >20 mg/L Zr. Although mortality was 100% at a higher dose of 100 mg/L Zr, this was attributed entirely to the effects of pH.

Description of key information

The LC50 (96 hr) in rainbow trout (Salmo gairdneri) was reported to be >20 mg/L Zr. Although mortality was 100% at a higher dose of 100 mg/L Zr, this was attributed entirely to the effects of pH.

The Zr4+ content of the anhydrous registered substance is ca 37%; using an assumed LC50 of 20 mg/l Zr 2 +, the product LC50 is estimated 54 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
54 mg/L

Additional information

A figure of 54 mg/l is used for the CSR and classification of the anhydrous oxynitrate