Ethylamine-boron trifluoride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable rerictions, no analytical measurements, exposure period of 8 days is still acceptable for this slowly growing cyanobacteria (should still be in log phase at the end of test)

Data source

Reference

Reference Type:

publication

Title:

Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Blaualgen (Microcystis aeroginosa) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest

Author:

Bringmann G, Kuehn R

Year:

1978

Bibliographic source:

Vom Wasser 50: 45-60

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 9

Version / remarks:

according to Brinkmann

Principles of method if other than guideline:

DIN 38412 part 9, according to Bringmann

GLP compliance:

no

Test material

Test material information

1

Reference substance name:

Ethylamine

EC Number:

200-834-7

EC Name:

Ethylamine

Cas Number:

75-04-7

Molecular formula:

C2H7N

IUPAC Name:

ethylamine

Test material form:

solid - liquid: aqueous solution

Details on test material:

neutralised

Specific details on test material used for the study:

70% aqueous solution, neutralised

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

a serial dillution is prepared by dilution of a 40 ml stock solution of known concentration with 40 ml bidest. water and further dillution in the same way 14 times

Test organisms

Test organisms (species):

Microcystis aeruginosa

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

8 d

Remarks on exposure duration:

shaking of the vessels once per day

Test conditions

Test temperature:

27°C

pH:

7.0

Nominal and measured concentrations:

serial dillution each 2 fold

Details on test conditions:

in vessel 5 ml dilluted substance + 5ml algae suspensio are added up to a volume of 50 ml
10 ml are transferred to the test vessel: (Kapsenberg-Kulturröhrchen, 18x180 mm)

Algae suspension
- Initial cells density was adjusted to an extintion equal to the Formazin Standard Extintion (for the monochromatic radiation Hg 578 nm (thickness of cuvette)) of TE/F/578 nm = 20

measured effect parameters
- Determination of cell concentrations: spectrophotometer: Extintion of the monochromatic radiation Hg 578 nm Hg
- if a change of colour or turbidity was observed, the radiation was adapted to Hg 691 nm or 436 nm

Reference substance (positive control):

no

Remarks:

report compairs 180 tests on over 60 substances

Results and discussion

Effect concentrations

Key result

Duration:

8 d

Dose descriptor:

other: TT: Toxic Threshold Concentration

Remarks:

corresponds to EC3 and can be regarded as NOEC

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Any other information on results incl. tables

toxicity of ethylamine to algae was determined in an 8 day study conducted according to Bringmann (DIN 38412 part 9) with cultures of the Microcystis aeruginosa. The TT (NOEC) value based on biomass was 1.3 mg/L (nominal).

Applicant's summary and conclusion

Conclusions:

ethylamine in neutralised solution shows medium toxicity to microcystis aeruginosa

Executive summary:

Test Organism: Microcystis aeruginosa

Test Type: Static

8 d TT (NOEC):  1.3 mg/L nominal ethylamine, neutralised

Endpoint(s) Effected: Biomass