Propanenitrile, 2-[bis(cyanomethyl)amino]-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test substance: MGDN
- Purity: 99.9 g/100g by 1 H-NMr spectroscopy; 99.5 area % by HPLC-fingerprint
- Chemical name: Propanenitrile, 2-[bis(cyanomethyl)amino]-
- Molecular formula: C7H8N4
- Molecular weight: 148.17 g/moL

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

Because the test substance is sufficiently soluble in water a stock solution was prepared. For this 2000 mg of the test substance were stirred in approximately 900mL demineralised water at room temperature until the test substance was dissolved completely. After that the pH value of the stock solution was measured and adjusted to 7.5 ± 0.5. Following this the stock solution was made up to 1 L with demineralised water. The stock sotution appeared colourless-clear.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Activated sludge from the municipal wastewater treatment plant of the city Mannheim/Baden Württemberg (Germany).
- lt was collected on 28 Jun 2006 from the aeration tank of the plant.
- The activated sludge Suspension was washed 3 times with tap water, sieved with a fine woven mesh (mesh size about 1 mm).
- This Suspension was adjusted to a concentration of 5.0 g/L dry weight and pre-aerated for 24 hours.
- After the pre-aeration the lost water of the suspension was replaced.
- An aliquot of this suspension was added to the test vesseis to obtain a sludge concentration of 1.0 g/L dry substance.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2°C

pH:

7.4

Nominal and measured concentrations:

- Test concentration (nominal): 250, 500, and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessels
- Fill volume: 250 mL

Reference substance (positive control):

yes

Remarks:

dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

290 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

620 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate