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EC number: 239-554-5 | CAS number: 15519-28-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-04-14 to 2006-04-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and Guideline compliant study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 1997
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Caesium carbonate
- EC Number:
- 208-591-9
- EC Name:
- Caesium carbonate
- Cas Number:
- 534-17-8
- Molecular formula:
- CH2O3.2Cs
- IUPAC Name:
- Cesium carbonate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Method
- Target gene:
- The Salmonella typhimurium strains are constructed to differentiate between base pair (TA 1535, TA 100) and frameshift (TA 1537, TA 98) mutations. The Escherichia coli WP2 uvrA detect mutagens that cause other base-pair substitutions.
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- Phenobarbital (PB) and ß-naphthoflavone (BNF) induced rat liver S 9-mix
- Test concentrations with justification for top dose:
- 3, 10 33, 11, 333, 1000, 3330, 5000 µg/plate
- Vehicle / solvent:
- Milli-Q water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- methylmethanesulfonate
- Details on test system and experimental conditions:
- An initial mutation test was performed. Bacteria were exposed to the test item, both in the presence and absence of an appropriate metabolic activation system. Freshly grown cultures were used. It were used Agarplates with 25 ml glucose agar medium. Agar plates for test with Salmonella typhimurium strains also con tained 12.5µg/plate biotin and 15µg/plate tryptophan.
The entire test consisted of non-activated and activated test conditions (without S9 Mix and with addition of S9 Mix) and each of them with the addition of negative and positive controls.
Top agar in top agar tubes was molten and heated to 45°C. Following solutions were addet to 3 ml top agar: 0.1 ml bacterial culture of one of the tester strains, 0.1 ml of a dilution (tests substance in Milli-Q-water and either 0.5 ml S9 mix (for activation assay) or 0.5 ml 0.1 M phosphate buffer (for non activation assay). Ingrdients were mixed and poured onto a selective agar plate.
After solidification the plates were inverted and incubated at 37 °C for at least 48 hours in the dark and revertante colonies (Histidin independent for Salmonella typhimurium and tryptophan independet for Escherichia coli) were counted manually or with a Protos model 50000 counter.
The colony numbers on the control, positive control and the test plates were determined, the mean values, standard deviations and the mutation rates were calculated. - Evaluation criteria:
- Cesium Carbonat is considered negativ (not mutagenic) if the total number of revertants in tester strain TA 100 is no greater than two times in the concurrent control, in tester strain TA 1535, TA1537, TA 98 or WP2uvrA is not greater than 3 times in concurrent control. Negative response shold be reproducible in one independentliy repeated experiment.
Cesium Carbonat is considered positve (mutagenic) if the total number of revertants in tester strain TA 100 is greater than two times in the concurrent control, in tester strain TA 1535, TA1537, TA 98 or WP2uvrA is not greater than 3 times in concurrent control. Positive response will be repeated, and reproducible in one independentliy repeated experiment. - Statistics:
- not applicable
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Dose range finding test was reported as a part of the first experiment of the muation assay.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test item was investigated for its mutagenic potential in vitro via an Ames assay with and without metabolic activation. Based on the results, Cesium carbonate is considered to be non-mutagenic. - Executive summary:
The mutagenic potential of cesium carbonat was investigated in five bacterial strains: Salmonella typhimurium (TA 98, TA 100, TA 1535, TA1537 and Escherichia coli WP2 uvrA), according to OECD Guideline No. 471 and EU Method B 13/14. The test item was investigated in vitro via an Ames assay with and without metabolic activation. Therefor concentrations up to 5000 µg Cesium carbonate/plate were tested in two independent experiments in absence and presence of S9 -mix.
Based on the results, the test item is considered to be non-mutagenic.The bacterial background lawn was not reduced at any tested concentration and no increase in the number of revertants was observed up on treatment with Cesium Carbonate. Negativ and the strain-specific positiv control values indicate that test conditions were adequate.
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