Poly(oxy-1,2-ethanediyl), alpha-[2-[bis(2-aminoethyl)methylammonion)ethyl]-, omega-hydroxy, N,N'-di-Limnanthes alba seed oil acyl derivatives, methyl sulfates (salts)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 2001 to February 2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Justification for type of information:

OECD 301F guideline study with no significant deviations from the method. Some detailed documentation was not available, such as measurement method, but generally most information was available and the test is reliable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Poly(oxy-1,2-ethanediyl), alpha-[2-[bis(2-aminoethyl)methylammonion)ethyl]-, omega-hydroxy, N,N'-di-Limnanthes alba seed oil acyl derivatives, methyl sulfates (salts)

EC Number:

629-704-1

Cas Number:

226995-92-2

Molecular formula:

The substance is a UVCB - no specific molecular weight or formula is available.

IUPAC Name:

Poly(oxy-1,2-ethanediyl), alpha-[2-[bis(2-aminoethyl)methylammonion)ethyl]-, omega-hydroxy, N,N'-di-Limnanthes alba seed oil acyl derivatives, methyl sulfates (salts)

Test material form:

liquid

Specific details on test material used for the study:

Batch No: CW-4896
Composition: PEG-2dimeadowfoamamidoethylmonium methosulfate 85-90% and hexylene glycol 10-15%
Supplier: BEC
Date received: 14 December 2001
State: hydrodispersible; ready to use
Storage: room temperature
Stability: sponsor guarantees stability to 12 June 2002

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge sampled from the domestic water treatment plant "Louis Fargue" in Bordeaux on 9 January 2002. The sludge was prepared by filtration through a 0.5 mm sieve, then decanted into a mineral medium to decrease the dissolved organic carbon content. The activated sludge was aerated for 6 days and in accordance with the laboratory's own standard operating procedure MOP 5409/03 70 66 07.

Physico-chemical properties of the inoculum were reported as:
Suspended solids: 3.1 g/l
pH: 5.5
Dissolved oxygen: 7.6 mg/l

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

10 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Two test substance concentrations were used at 10 and 20 mg/l. An inoculum blank control and a positive control (sodium benzoate) were also established to measure the experimental conditions. The study was run in the dark at 20 degrees C +/- 1 degree C. Two replicates of each test substance concentration were used.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

No data

Test performance:

The test met the validity criteria for the following parameters:
- oxygen uptake (< 60 mg/l);
- variation between the two 10 mg/l replicates was < 20% at the end of the test
- reference substance showed biodegradability > 60% within 14 days
- 10 mg/l toxicity control biodegradation was > 25% within 14 days

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Remarks:

10 mg/l test substance

Value:

21.1

Sampling time:

28 d

Remarks on result:

other: not readily biodegradable

Remarks:

10 mg/l test substance

% Degradation 2

Parameter:

% degradation (O2 consumption)

Remarks:

20 mg/l test substance

Value:

8

Sampling time:

28 d

Remarks on result:

other: not readily biodegradable

Remarks:

20 mg/l test substance

Details on results:

The test substance as 10 mg/l is relied upon as the key result, which reported biodegradation at 21.1% after 28 days (average of two replicates). The test concentration at 20 mg/l is considered by the laboratory to have indicated a slight toxic effect at this concentration and the resulting biodegradation of 8% (average of two replicates) is not relied upon for further assessment.

BOD5 / COD results

Results with reference substance:

The positive controls (sodium benzoate) were reported as 77.3% and 57.1% in the 10 mgl and 20 mg/l, respectively, at day 14.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test substance (10 mg/l) was incubated with domestic activated sludge for 28 days and achieved 21.1% biodegradation. The substance is not classified as readily biodegradable, but some degradation did occur.

Executive summary:

The biodegradation of the test substance (at 10 mg/l) was assessed in a reliable OECD 301F Guideline study through the measurement of oxygen consumption when mixed with domestic activated sludge. After 28 days test duration, the test substance had degraded by 21.1% and is considered to be not readily biodegradable, although some degradation did occur.