Hydrolyzed Quinoa

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

Due to the lack of toxicity observed in preliminary range-finding tests (0.01 to 1000 mg/l concentrations tested) OECD Limit Tests were conducted using direct addition of material.
100 mg/l

Test organisms (species):

Daphnia magna

Details on test organisms:

SPECIES: Daphnia magna
ACCLIMATION/TEST WATER: Hard Syntetic Freshwater
FEEDING PRIOR TO TEST: YCT + Selenastrum capricornutum
FEEDING DURING TEST: None -YCT + Selenastrum 2+ h before test
SOURCE: CI Stock cultures
ACCLIMATION TEMP (°C ): 20
BROOD RELEASE START DATE & TIME: 3/30/18 16:00
BROOD RELEASE END DATE & TIME: 3/31/18 10:30
DATE/TIME WATER ADDED: 3/31/18 13:59
DATE/TIME ANIMALS ADDED: 3/31/18 14:17
ANIMAL AGE WINDOW: 18h 30m
MAX AGE AT TEST START: 22h 17m

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19 - 21

pH:

8.00 - 8.28

Dissolved oxygen:

7.2 - 8.9 mg/L

Conductivity:

568 - 579 uS/cm

Reference substance (positive control):

yes

Remarks:

KCl

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was greater than the test concentration of 100 mg/l.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrolyzed Quinoa

EC Number:

825-815-6

Cas Number:

227025-12-9

IUPAC Name:

Hydrolyzed Quinoa

Constituent 2

Reference substance name:

Water

EC Number:

231-791-2

EC Name:

Water

Cas Number:

7732-18-5

Molecular formula:

H2O

IUPAC Name:

Water

Test material form:

liquid

Details on test material:

dark clear liquid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

Test chamber: 250 ml glass flask
Test solution vol. (25 ml min): 100 ml:
Photoperiod: 24L:0D
Shaker Table Setting (rpm): 100

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

SPECIES: Selenastrum capricornutum (Pseodokirchneriella subcapitata)
TEST WATER: AAP
ILLUMINATION (lux): 450
LIGHT METER NO.: 24
ALGAE SOURCE: CBI Stock cultures (U. Texas)
ACCLIMATION TEMP (°C ): 24
A. INOC. CONC. COUNT (cells/ml x 10000): 510.0
B. INOCULATION VOLUME (ml): 1.0
INITIAL CONC. (cells/ml)(A x B/100): 51000.0

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

25°C

pH:

7.60 - 10.24

Nominal and measured concentrations:

100 mg/L nominal

Reference substance (positive control):

yes

Remarks:

KCl

Results and discussion

Effect concentrations

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Due to the limited solubility of the OECD Limit Tests were conducted using direct addition ofmaterial. Limit Tests were conducted. Sufficient replicates were tested (6 replicates for algae) to allow for hypothesis testing and confident demonstration that the EC50/LC50 was greater than the test concentration of 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50/LC50 was greater than the test concentration of 100 mg/l