Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
white light tan powder, purity = 99.8%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
samples were withdrawn from the treatment solutions at 0 and 48 h;

Test solutions

Vehicle:
no
Details on test solutions:
test solution prepared by transferring wighted amounts of A-172245.0 into the glass beakers. The containers were made up to 100 mL with reconstituted water in order to obtain the final concentration of 100 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Fill in what you can

TEST ORGANISM
- Common name:
- Strain:
- Source:
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
The obersavation period was 48 hours. observation for immobilization was made at 24 and 48 hours. Daphnia was considered immobile if not able to swim within 15 seconds of gentle agitation of the test container.

Test conditions

Hardness:
200-250 mg/l
Test temperature:
temperature was monitored
pH:
pH was monitored
Nominal and measured concentrations:
Nominal concentration = 100 mg/l

measured conc = 102.17 mg/l @ 0 hours
measured conc = 101.63 mg/l @ 48 hours
Details on test conditions:
during the test, the animals were kept in the dark

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of A-172245.0 @24 hours and @ 48 hours was higher than 100mg/l