2,5-dioxopyrrolidin-1-yl N-{[methyl[[2-(1-methylethyl)-4-thiazolyl]methyl]amino]carbonyl}-.sc.l.sc.-valinate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 May - 07 Jun, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under OECD method and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Version / remarks:

Part IV

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

424-660-8

EC Name:

-

Cas Number:

224631-15-6

Molecular formula:

Hill formula: C18H26N4O5S CAS formula: C18H26N4O5S

IUPAC Name:

2,5-dioxopyrrolidin-1-yl (2S)-3-methyl-2-{[methyl({[2-(propan-2-yl)-1,3-thiazol-4-yl]methyl})carbamoyl]amino}butanoate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

29 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

A pre-determined amount of test material was added to a liquid medium to give duplicate bioreactors containing 10mg and 20 mg Organic Carbon (OC). These were inoculated with micro-organisms from activated sludge and aerated at 20-25°C. A ny Co2 released as a result of biodegredation trapped and monitored over 29 days.. The total CO2 produced was calculated as a percentage of the total CO2 that the test material,, based on its carbon content, could have theoretically produced.

Reference substance

Reference substance:

other: Sodium benzoate

Results and discussion

Preliminary study:

Under the strict conditions of the test, A-133816.0 is determined not to be readily biodegadeable. A-133816.0 is determined to be readily biodegadable at a concentration of 20mg/L
There was no inhibitory effects to the micro-organisms contained in the sludge innoculum at a concentration of ca. 37.8mg/L

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

20.83

Sampling time:

20 d

Remarks on result:

other: 10mg/l sample

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

51.18

Sampling time:

20 d

Remarks on result:

other: 20mg/L sample

BOD5 / COD results

Results with reference substance:

Sodium benzoate attained 76.16% biodegredation within 20 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other:

Conclusions:

Under the strict conditions of the test, A-133816.0 is determined not to be readily biodegadeable. A-133816.0 is determined to be readily biodegadable at a concentration of 20mg/L
There was no inhibitory effects to the micro-organisms contained in the sludge innoculum at a concentration of ca. 37.8mg/L