Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27 Mar - 30 Mar, 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Done under GLP and OECD methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
Method C3, Directive 92/69/EEC
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol
EC Number:
414-810-0
EC Name:
A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol
Cas Number:
144163-85-9
Molecular formula:
Empirical formula: C25H35N2O5
IUPAC Name:
butanedioic acid; propan-2-ol; bis(tert-butyl N-[(4R)-5-amino-4-hydroxy-1,6-diphenylhexan-2-yl]carbamate)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
Nominal test concentrations: 0.10, 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L
Mean measured test concentrations: 0.082, 0.17, 0.41, 0.70, 2.3 and 4.5 mg/L
Each test solution was prepared in triplicate. One untreated control was also used.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, c/o Freshwater Biological Association, Cumbria, England.

Study design

Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 1°C
Nominal and measured concentrations:
Nominal test concentrations: 0.10, 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L
Mean measured test concentrations: 0.082, 0.17, 0.41, 0.70, 2.3 and 4.5 mg/L
Each test solution was prepared in triplicate. One untreated control was also used.
Details on test conditions:
Algae were cultured for 72hrs under continuous illumination of approx. 7,000 lux in an orbital shaker at 120 cycles /minute.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
other: EbC50 (based on comparison of area under growth curves).
Effect conc.:
0.37 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95%CL: 0.27 - 0.51 mg/L
Duration:
72 h
Dose descriptor:
other: ErC50 (comparison of growth rates)
Effect conc.:
0.72 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95%CL: 0.58 - 0.88 mg/L
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
0.17 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
EbC50 (72 hr): 0.37 mg/L
ErC50 (0 - 72 hr): 0.72 mg/L
NOEL: 0.17 mg/L
Results with reference substance (positive control):
Mean cell density of control @ 0 hrs: 1.29 x 10 exp 4
Mean cell density of control @ 72 hrs: 7.43 x 10 exp 5

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EbC50 (72 hr): 0.37 mg/L
ErC50 (0 - 72 hr): 0.72 mg/L
NOEL: 0.17 mg/L