Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 20-22 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Test concentrations were verified by chemical analysis. water samples (~50 ml in duplicate) were taken from the control and each expsoure level (replicates pooled) at 0 hours and 48 hours.

Test solutions

Vehicle:
no
Details on test solutions:
seven test concetrations plus one control, each in duplicate (20 animals per test group)

Ten 1st instar Daphnia were placed at random in each glass jar containing 200 ml of prepared test medium or diluent media only, as appropiate, to give a loading of 20 ml test solution per organism.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:

-Common name: ITChA, France
- Source: IRChA, France

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
groups of twenty 1st instar daphnia (;es than 24 hours old) were exposed to seven concentrations of A-88820.605 dispersed in Elendt M7 medium. The incidence of immobilisation was recorded for each test and control group at 24 hours and at 48 hours.

Test conditions

Test temperature:
20 oC
Nominal and measured concentrations:
nominal test concentratons: 1.0, 2.2, 4.6, 10, 22, 46 and 100 mg/l
Measured test concentrations: 0.95, 2.0, 4.6, 9.5, 20, 44 and 100 mg/l
Details on test conditions:
Photoperiod: 16 hous light, 8 hours dark

Reference substance (positive control):
yes
Remarks:
Potassium dichromate.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 17 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CL: 13-22 mg/l
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 9.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 7.1-12 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48 hour EC50 (immobilisation) value for A-88820.605 with Daphnia magna is 9.2 mg/l