A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 20-22 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol

EC Number:

414-810-0

EC Name:

A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol

Cas Number:

144163-85-9

Molecular formula:

Empirical formula: C25H35N2O5

IUPAC Name:

butanedioic acid; propan-2-ol; bis(tert-butyl N-[(4R)-5-amino-4-hydroxy-1,6-diphenylhexan-2-yl]carbamate)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Test concentrations were verified by chemical analysis. water samples (~50 ml in duplicate) were taken from the control and each expsoure level (replicates pooled) at 0 hours and 48 hours.

Test solutions

Vehicle:

no

Details on test solutions:

seven test concetrations plus one control, each in duplicate (20 animals per test group)

Ten 1st instar Daphnia were placed at random in each glass jar containing 200 ml of prepared test medium or diluent media only, as appropiate, to give a loading of 20 ml test solution per organism.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

-Common name: ITChA, France
- Source: IRChA, France

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

groups of twenty 1st instar daphnia (;es than 24 hours old) were exposed to seven concentrations of A-88820.605 dispersed in Elendt M7 medium. The incidence of immobilisation was recorded for each test and control group at 24 hours and at 48 hours.

Test conditions

Test temperature:

20 oC

Nominal and measured concentrations:

nominal test concentratons: 1.0, 2.2, 4.6, 10, 22, 46 and 100 mg/l
Measured test concentrations: 0.95, 2.0, 4.6, 9.5, 20, 44 and 100 mg/l

Details on test conditions:

Photoperiod: 16 hous light, 8 hours dark

Reference substance (positive control):

yes

Remarks:

Potassium dichromate.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 17 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CL: 13-22 mg/l

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 9.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 7.1-12 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 (immobilisation) value for A-88820.605 with Daphnia magna is 9.2 mg/l