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EC number: 947-780-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Remarks:
- In vivo study
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Terpenes and terpenoids, turpentine oil, alpha pinene fraction oligomers
- IUPAC Name:
- Terpenes and terpenoids, turpentine oil, alpha pinene fraction oligomers
- Reference substance name:
- Terpenes and terpenoids, turpentine-oil, α-pinene fraction, oligomers
- EC Number:
- 500-245-8
- EC Name:
- Terpenes and terpenoids, turpentine-oil, α-pinene fraction, oligomers
- Cas Number:
- 70750-57-1
- Test material form:
- liquid: viscous
- Details on test material:
- - Name of test material (as cited in study report): Terpenes and terpenoids, turpentine oil, alpha pinene fraction
- Substance type: UVCB
- Physical state: extremely viscous pale amber coloured liquid
- Analytical purity: 100%
- Lot/batch No.: 0910002624
- Expiration date of the lot/batch:indefinite
- Storage condition of test material: room temperature in the dark
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Test Material: Terpenes and terpenoids, turpentine oil, alpha pinene fraction oligomers
Description: extremely viscous fraction oligomers
CAS 70750-57-1
Batch Number: 0910002624
Purity: 100%
Date received: 14 March
Expiration date: Indefintive
Storage: room temperature in the dark
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Laboratories Ltd. Oxon, UK
- Age at study initiation: 8-12 weeks old
- Weight at study initiation: 15-23 g
- Housing: The animals were individually housed in suspended solid-floor polypropylene cages furnished with softwood woodflakes.
- Diet (e.g. ad libitum): free access
- Water (e.g. ad libitum): free access
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature : 19-25°C
- Humidity: 30-70%
- Air changes (per hr):
- Photoperiod: 12h dark, 12 h light
Study design: in vivo (LLNA)
- Vehicle:
- other: butanone
- Concentration:
- Mice were treated by daily application of 25 μl of the test material at concentration of 25% or 50% w/w in butanone.
- No. of animals per dose:
- 4
- Details on study design:
- The preliminary test showed no systemic toxicity or excessive local skin irritation at the highest suitable concetration. Daily application of 25µl of the appropriate concentration of the test item was applied to the dorsal surface of each ear for three consecutive days. Five days following the first topical application all mice were injected via the tail vein with 250 µl of phosphate buffered saline (PBS) containing 3H-methyl thymidine giving a total of 20 µCi to each mouse.
- Statistics:
- The proliferation response of lymph node was expressed as the number of radioactive disintegrations per minutes per lymph node and as the ratio of 3HTdR incorporation into lymph node cells of the test nodes relative to that recorded for the control nodes. (SI).
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- SI
- Value:
- 1.31
- Remarks on result:
- other: Concentration 5% w/w in butanone
- Key result
- Parameter:
- SI
- Value:
- 1.04
- Remarks on result:
- other: 10% w/w in butanone
- Key result
- Parameter:
- SI
- Value:
- 2.94
- Remarks on result:
- other: 25% w/w in butanone
- Cellular proliferation data / Observations:
- Clinical Observation: Mild (very slight) erythema on the ears was noted in animals treated with the test item at a concetration of 25% w/W in butanone. There were no deaths. No signs of systemic toxicity were noted in the test or control animals during the test.
Body weight: One animal, treated with the test ite, at concentration of 10% w/w in butanone, showed a slightly greater than expected bodyweight loss (3g) over the test period. Bodyweight changes of the remaining test animals between Day 1 and Day 6 were comparable to those observed in the corresponding control group animals.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test item is considered to be a non-sensitiser under the condition of this test.
- Executive summary:
A LLNA test was performed to investigate the skin sensitisation potential of Terpenes and terpenoids, turpentine oil, alpha pinene fraction oligomers. The study was performed according to the OECD 429 and under GLP conditions. The test item was applied onto the dorsal surface of the ear of CBA/Ca strain female mice. Following a preliminary screening test in which no systemic toxicity was recorded at concentration of 25% w/w, this concentration was selected as the highest dose investigated. The results were expressed as Simulation Index (SI) and under the condition of the study, the test item was considered to be a non-sensitiser.
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