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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2019
Justification for type of information:
Please refer to read-across justification attached in IUCLID Section 13.
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
and yield
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
and yield
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
and yield
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
and yield
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
and yield

Description of key information

The 72-h NOEC based on growth rate and yield for the source test item was determined to be 100 mg/L, while the 72-h LOEC based on growth rate and yield was determined to be > 100 mg/L. The 72-h EC10, EC20 and EC50 values were determined to be > 100 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for freshwater algae:
100 mg/L

Additional information

No data on the substance itself is available. Therefore, read-across was applied to the source substance Phosphoric acid, 2-ethylhexyl ester, sodium salt (CAS 68186-64-1). This approach is considered appropriate since both substances are UVCB substances consisting of a mono-, bis- and tris- substituted phosphate with C-chains (4 - 6 C), only the cation is different. In the target substance the cation is ammonium, while the source substance has sodium.

The effect of the test item on the growth of a unicellular green algal species Raphidocelis subcapitata was determined according to OECD 201 and Regulation (EC) No. 226/2016 Method C.3 under GLP. The study was conducted under static conditions with an initial cell density of 1E4 cells/mL for a period of 72 hours. A limit test with one test concentration of 100 mg/L, and control was performed. The measured concentrations of the test item at the start and at the end of the test were in the range of 95 - 102 % of the nominal concentration. The measured concentrations deviated not more than ± 20 % from the nominal concentration, therefore, the nominal concentration was used to determine the biological results. The EC50-values for inhibition of growth rate (ErC50) and for inhibition of yield (EyC50) were >100 mg/L. The ErC10 and EyC10 values were determined to be >100 mg/L. The NOEC was determined to be 100 mg/L.

Based on the structural similarities between the source and the target substance, the target substance is also considered to not be toxic to aquatic algae up to the limit concentration.