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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Link to relevant study record(s)

Description of key information

No terrestrial ecotoxicity tests have been performed with Sodium diisobutylnaphthalenesulphonate (CAS no 27213-90-7). The results from the structural analogue Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt is therefore read across.

Effects of Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida (Annelida, Lumbricidae) were determined according to OECD 222 (2016).       

The study was conducted under static conditions over 8 weeks with the test item concentrations 15.625 – 31.25 – 62.5 – 125 – 250 – 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW.

Each application rate was mixed into artificial soil containing 5 % peat. A control using untreated artificial soil was tested under the same conditions as the test item treatments.

After 28 days of exposure in soil, neither evident earthworm mortalities (< 10%) nor pathological symptoms or changes in the behaviour of adult earthworms were observed in the control or all test item concentrations. There were no statistically significant differences in earthworm body weight gains in the test item concentrations 15.625 to 250 mg test item/kg soil dry weight compared to the control. However, at the test item concentration of 500 mg/kg the earthworm body weight gain was statistically significantly reduced. After a further four weeks, the reproduction rate (average number of juveniles produced) was 127 in the control and ranged from 28 to 139 in the test item treatment rates. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.625 to 250 mg test item /kg SDW compared to the control. However, at the test item concentration 500 mg test item/kg the earthworm reproduction was statistically significantly reduced.

Overall, the EC10of the test item concerning reproduction was determined to be 260 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW. The EC50-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.  

All validity criteria recommended by the test guidelines were fulfilled.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
250 mg/kg soil dw

Additional information

The EC50-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.

The NOEC-value for mortality was 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.

No mortality was observed at 1000 mg/kg SDW in the 14 day (non-GLP) range finder.