Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-930-1 | CAS number: -
Bernel Ester TCC was assayed for mutation in five histidine-requiring strains (TA98, TA100, TA1535, TA1537 and TA102) of Salmonella typhimurium, both in the absence and in the presence of metabolic activation by an Aroclor 1254-induced rat liver post-mitochondrial fraction (S-9), in two separate experiments.
All Bernel Ester TCC treatments in this study were performed using formulations prepared in N-methyl-2-pyrrolidone (NMP).
Mutation Experiment 1 treatments of all the tester strains were performed in the absence and in the presence of S-9, using final concentrations of Bernel Ester TCC at 5, 16, 50, 160, 500, 1600 and 5000 μg/plate, plus vehicle and positive controls. Following these treatments, evidence of toxicity was observed at 5000 μg/plate in all strains in the absence and presence of S-9.
Mutation Experiment 2 treatments of all the tester strains were performed in the absence and in the presence of S-9. The maximum test concentration of 5000 μg/plate was retained for all strains. Narrowed concentration intervals were employed covering the range 160-5000 μg/plate except strain TA98 which was also treated at 5, 16 and 50 μg/plate in the absence and presence of S-9 in order to investigate reproducibility of increases in revertant numbers observed in the absence of S-9 in Mutation Experiment 1. In addition, all treatments in the presence of S-9 were further modified by the inclusion of a pre incubation step. In this way, it was hoped to increase the range of mutagenic chemicals that could be detected using this assay system. Following these treatments, no evidence of toxicity was observed.
Precipitation was observed on all Mutation Experiment 2 test plates at 2500 μg/plate and above in the absence and presence of S-9.
Vehicle and positive control treatments were included for all strains in both experiments. The mean numbers of revertant colonies fell within acceptable ranges for vehicle control treatments, and were elevated by positive control treatments.
Although some ≥2-fold increases in revertant numbers were observed following Bernel Ester TCC treatments of strain TA98 in the absence of S-9 at 50 and 1600 μg/plate in Mutation Experiment 1, these were not concentration-related or reproducible and were of insufficient magnitude to be considered as clear evidence of mutagenic activity in this assay system.
It was concluded that Bernel Ester TCC did not induce mutation in five histidine-requiring strains (TA98, TA100, TA1535, TA1537 and TA102) of Salmonella typhimurium when tested under the conditions of this study. These conditions included treatments at concentrations up to 5000 μg/plate (the maximum recommended concentration according to current regulatory guidelines), in the absence and in the presence of a rat liver metabolic activation system (S-9).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again