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EC number: 947-930-1 | CAS number: -
The ready biodegradability of Bernel Ester TCC was assessed by measurement of carbon dioxide (CO2) evolution under standard conditions. The procedure followed was that of OECD Guideline 301B, Ready Biodegradability (Adopted 1981, Revised 1992).
The test substance, Bernel Ester TCC, was added to the test system directly. Buffered mineral salts medium was added to give a test substance concentration equivalent to 15 mg organic carbon/L. The medium was inoculated with microorganisms derived from a sample of activated sludge not previously intentionally exposed to the test substance. Test vessels were incubated in darkness at 22 ± 2°C for 28 days and their contents continuously sparged with a supply of CO2-free air. The exhaust air from each vessel was passed through a series of traps containing a barium hydroxide solution to trap evolved CO2.
At regular intervals during the incubation, traps were detached and their contents titrated with hydrochloric acid to determine the quantity of CO2 evolved from the respective test vessels. At the end of incubation, 28 days, the test vessel contents were acidified to release any residual CO2 that may have remained in solution. Titration of the traps was performed following overnight aeration.
The procedure and the activity of the inoculum were checked by measuring the CO2 evolved from vessels containing a reference substance, sodium benzoate. An additional vessel containing a combination of the test and reference substances served as a toxicity control to assess whether the test substance was inhibitory to biodegradation at the test concentration. Two blank control vessels were also prepared containing inoculated medium only. The results of these vessels were used to check the validity of the test and to correct the evolved CO2 values.
Bernel Ester TCC showed < 10% biodegradation during the incubation. As a result, Bernel Ester TCC cannot be considered readily biodegradable.
The mean total CO2 production in the blank control vessels was 43.3 mg/L at the end of the test, satisfying the validity criterion of < 120 mg/L.
Mean biodegradation of the reference substance had reached 60% by Day 7 and had reached a mean of 78 % by the end of the incubation phase on Day 28 and 79% at the end of the test. The rate of biodegradation of the reference substance in the presence of Bernel Ester TCC did exceed the 25% recommended by the guidance document on day 2 (32%) and reached a maximum of 37%, therefore the test was considered to be valid. The observed values are noted to be low, however this seems likely to be indicative of the variability of the biological nature of the test system.
The inorganic carbon content of the test medium was determined to be 1.62 mg carbon/L, corresponding to 3.6 % satisfying the validity criterion of < 5%.
All validity criteria were satisfied, the blank controls and reference vessels performed as expected, and the results of this study are therefore considered to be valid.
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