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EC number: 947-979-9 | CAS number: -
The mixture of 50 µL test item per 1 mL MTT medium showed no reduction of MTT as compared to the solvent. The mixture turned to dark red, but not blue/purple.
Although the absorption spectra of the mixture showed a peak at 555 nm with an OD of 2.5296, the NSMTT was not determined since the viability of the test item treated tissues (TM) was below 60%.
The mixture of 50 µL test item per 1 mL A. dest. and per 2 mL isopropanol showed no colouring as compared to the solvent.Therefore, NSC equaled 0%.
Ocular irritation potential of the test item was predicted from the relative mean tissue viabilities compared to the negative control tissues concurrently treated with Aqua dest.
The test item showed irritant effects. The mean relative tissue viability (% negative control) was ≤ 60% (10.7%).
The controls confirmed the validity of the study. The mean absolute OD570of the two negative control tissues was> 0.8 and < 2.5 (2.082). The mean relative tissue viability (% negative control) of the positive control was < 50% (32.5%). The maximum inter tissue difference of replicate tissues of all dose groups was < 20% (0.1 – 9.4%).
This study is classified as acceptable. This study satisfies the requirement for OECD Guideline for the Testing of Chemicals No. 492: (Reconstructed human Cornea-like Epithelium (RhCE) test method).
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