Dihydrogen bis[monoperoxyphthalato(2-)-O1,OO1]magnesate(2-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Guideline study (not GLP compliant) without analytical monitoring. These are only a minor shortcoming and do not affect the overall significance of the findings.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Remarks:

GLP was not mandatory at this time, but report has been reviewed by the testing facilities Quality Assurance Unit

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout (Oncorhynchus mykiss Walbaum 1792, former scientific name: Salmo gardneri)
- Source: Bibury Trout Farm, Gloucestershire, Great Britain
- Length at study initiation: 23.9 +/- 0.13 mm
- Weight at study initiation: 158 +/- 34 mg

ACCLIMATION
- Type and amount of food during acclimation: Mixed diet of hens' eggs and proprietary trout feed (B.P. Nutrition U.K. Ltd., Mainstream trout diet)

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

extended up to 168 hours

Hardness:

250 - 300 mg/l as calcium carbonate

Test temperature:

13.5 - 15 °C

pH:

6.7 – 7.6

Dissolved oxygen:

7.9 – 9.5 mg/L

Nominal and measured concentrations:

Control, 8.03, 12.8, 20.0, 31.7, 52.2, 80.3, 128, 200, 317, 522, 803, 1284, 2000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 2 L borosilicate glass flasks
- Renewal rate of test solution: 20 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deep well in the Hertfordshire chalk

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 13:11 hours light:dark cycle

EFFECT PARAMETERS MEASURED:
After 24, 48, 96 and 168 hours mortality, at this time also pH and oxygen content were recorded

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

31.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CI: 43.2-76.6 mg/L

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

128.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

Calculation of median lethal concentration (LC50) after 12, 24, 48, 96 and 168 hours, using methods described by Litchfield JT (1949) and Litchfield LT & Wilcoxon F (1949)

Sublethal observations / clinical signs:

 

Nominal [mg/L]	Mortality after 96 h [%]	Mortality after 168 h [%]
Control	0	0
12.8	0	0
20	0	20
31.7	0	90
52.2	60	100
80.3	80	100
128.4	100	100

In concentrations of 200 mg/L and higher no fish survived the first day, therefore these results are not listed in the table above.

Validity criteria fulfilled:

yes

Conclusions:

The test results indicate that MMPP is acutely harmful towards fish

Executive summary:

The acute toxicity of MMPP towards fish was performed according to the OECD guideline 203 (1984). Rainbow trout O. mykiss was used as test organism.

Ten fish were exposed to each of the 13 concentrations of the test substance between 8.3 and 2000 mg/L and to a control. The test was performed at 15 °C. Test duration was 168 hours and survival was assessed troughout this period in variable intervals. pH and dissolved oxygen concentrations were also measured.

H48 BLEACH was found to be toxic towards fish. A clear concentration-response relationship can be deduced from the study results. The numerical results for acute toxicity are given below. Additionally, after 168 hours an LC₅₀ of 38.5 mg/L has been obtained.

Analytical monitoring of test concentration was not performed. Volatility and adsorption of MMPP from aqueous solutions can be neglected and therefore these properties have no influence on the result. However, MMPP hydrolyses in aqueous solution and MMPP is regarded as readily biodegradable; both effects may reduce the nominal concentration. Nevertheless, an analytical monitoring is not necessary, as the aqueous solution of MMPP reflects the emission of hydrolyses products to aquatic organisms. Furthermore, the biodegradability is assumed to have only minor effects on the results due to the relatively short test duration. The concentration in aqueous solution is assumed to be sufficiently stable under the test conditions due to the analytical results shown in the algae growth inhibition test.

All validity criteria according the test guideline OECD 203 are met within the study.

Description of key information

The acute toxicity of MMPP towards fish was performed according to the OECD guideline 203 (1984). Rainbow troutO. mykisswas used as test organism.

Test duration was 168 hours and survival was assessed troughout this period in variable intervals. pH and dissolved oxygen concentrations were also measured.

H48 BLEACH was found to be toxic towards fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

56 mg/L