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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001-01-25 to 2001-03-12
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
-
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
α-acetyl-γ-butyrolactone
EC Number:
208-235-2
EC Name:
α-acetyl-γ-butyrolactone
Cas Number:
517-23-7
Molecular formula:
C6H8O3
IUPAC Name:
α-acetyl-γ-butyrolactone

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 d

Results and discussion

Effect concentrations
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
300 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 120 - 800 mg/l

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of 2-acetylbutyrolactone to Daphnia magna was determined in a 24 hour static test according to OECD guideline 202. Based on mean measured concentration, the 24-h EC50 was determined to be 300 mg/L with a 95% confidence interval ranging from 120 to 800 mg/L. The results of the test can be considered reliable without restriction.
Executive summary:

A range-finding test was followed by a final EC50 test performed under static conditions for 24 hours of exposure. The range-finding test showed a 24 EC50 value between 100 and 1000 mg/l.

In the final EC50 test Daphnia were therefore exposed for a maximum of 24 hours to nine different concentrations (5000, 2500, 1250, 625, 320, 160, 80, 40, 20, 0 mg/l)

The study included a blank-control. The test was performed in quadruplicate with 5 daphnia

per vessel. The 24h EC50 was determined to > 300 mg/l (95% CL: 120 - 800 mg/l)

24 h EC50 (reference substance) = 1.82 mg/l