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Ecotoxicological information

Toxicity to terrestrial plants

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Description of key information

TS-ED 532 was tested according to OECD guideline 208 “Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test” to spring barley, Hordeum vulgare, oil seed rape, Brassica napus and tomato, Lycopersicon esculentum, in the standard sandy test soil LUFA 2.1. Barley was the most sensitive species to TS-ED 532. LOEC was 250 mg/kg (nominal) for rape and tomato and 25 mg/kg (nominal) for barley based on plant dry weight effect. No effects were observed for seed emergence and survival of emerging plants (postemergence). EC50 estimates were between 100 to 200 mg/kg of the initial concentration except for barley where EC50 was 12.5 mg/kg. 

Key value for chemical safety assessment

Short-term EC50 or LC50 for terrestrial plants:
12.5 mg/kg soil dw
Long-term EC10, LC10 or NOEC for terrestrial plants:
25 mg/kg soil dw

Additional information

TS-ED 532 was tested according to OECD guideline 208 “Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test” for effects to the monocotyledon spring barley (Hordeum vulgare), and the dicotyledons oil seed rape (Brassica napus) and tomato (Lycopersicon esculentum) in the standard sandy test soil LUFA 2.1. The plant test comprised a short-term (an observation period of 7-10 days) range finding test with two replicates of each treatment, lasting 2 weeks (assuming a germination period lasting maximum 4-7 days), and a definitive test with an observation period of 22 days after 50% emergence of seedlings in the water control group (negative control). A positive control group (boric acid) was included. The endpoints measured include % emergence, shoot- and root-length for the range-finding test, and % emergence, survival and shoot biomass (dry weight) for the definitive tests.

NOEC and LOEC, mg/kg, in terms of nominal, initial and Time Weighed Average (TWA) concentrations toxicity endpoints for the three plant species, were determined. EC10 and EC50 for the three plant species was estimated by modelling of the three concentration measures: nominal, initial analytical concentrations and TWA concentrations.

LOEC was 250 mg/kg (nominal) for rape and tomato and 25 mg/kg (nominal) for barley based on plant dry weight effect. No effects were observed for seed emergence and survival of emerging plants (post emergence). EC50 estimates were between 100 to 200 mg/kg of the initial concentration except for barley where EC50 was 12.5 mg/kg.