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EC number: 226-195-4
CAS number: 5324-84-5
Change of Opacity
Irritation Score (IVIS)
OECD 437 (2017) - The Bovine Corneal
Opacity and Permeability (BCOP) test was conducted using sodium octane
1-sulphonate in accordance with OECD Guideline 437 "Bovine Corneal
Opacity and Permeability Test Method for Identifying i) Chemicals
Inducing Serious Eye Damage and ii) Chemicals Not Requiring
Classification for Eye Irritation or Serious Eye Damage" (2013).
Test item was dissolved in physiological
saline (0.9 % NaCl) to form a 20 % solution concentration. Triplicate
corneas had their opacity measured and were incubated in the test
solution for 4 hours ± 5 mins before being rinsed with minimal essential
medium (with phenol red). After being further rinsed with RPMI (without
phenol red) the opacity of each cornea was again measured. The
permeability of the corneas was determined by measuring RPMI solution
containing sodium fluorescein that has passed through the cornea
membrane, after being suspended for 90 mins in a horizontal position,
using a spectrophotometer. A
negative and positive control group, each containing 3 corneas, were
also prepared and treated using the same methodology.
the test item, each of the treated corneas showed complete opacity of
the tissue and whitish discolouration of the corneas. The
negative and positive control values were in concordance with historical
values and all guideline validity criteria were satisfied.
the conditions of this study, and with an IVIS score of 208.5, sodium
octane-1-sulphonate showed the potential to induce serious eye damage. According
to the guideline evaluation criteria, the test item is classified as UN
GHS Category 1 for serious eye damage/ eye irritation.
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