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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
other: ANFOR T90-303 Juin 1978
Version / remarks:
Essais des eaux. Détermination de la toxicité aigüe d'une substance vis à vis de Brachydanio Rerio (essai statique).
Deviations:
no
Qualifier:
according to
Guideline:
ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
Deviations:
no
GLP compliance:
not specified
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: not specified
- Expiration date of the lot/batch: not specified
- Purity test date: not specified

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: not applicable
- Specific activity: not applicable
- Locations of the label: not applicable
- Expiration date of radiochemical substance: not applicable

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability under test conditions: not specified
- Solubility and stability of the test substance in the solvent/vehicle: not specified
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not specified

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: according to the reported guideline
- Preliminary purification step (if any): -
- Final dilution of a dissolved solid, stock liquid or gel: -
- Final preparation of a solid: -

FORM AS APPLIED IN THE TEST (if different from that of starting material) -

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable) -

OTHER SPECIFICS: -
Analytical monitoring:
not specified
Details on sampling:
according to the reported guideline
Vehicle:
not specified
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Strain: Hamilton Buchanan
- Source: not specified
- Age at study initiation (mean and range, SD): not specified
- Length at study initiation (length definition, mean, range and SD): 2.5 ± 0.2 cm (without the tail)
- Weight at study initiation (mean and range, SD): not specified
- Method of breeding: not specified
- Maintenance of the brood fish: Fishes are bought at maturity and then raised in the laboratory in an aquarium with a capacity of 100 liters containing 70 liters of "aged" water.

ACCLIMATION
- Acclimation period: The acclimation period lasts as a minimum two weeks before use for the tests
- Acclimation conditions (same as test or not): not specified
- Type and amount of food during acclimation: not specified
- Feeding frequency during acclimation: not specified
- Health during acclimation (any mortality observed): not specified

QUARANTINE (wild caught)
- Duration: not specified
- Health/mortality: not specified

FEEDING DURING TEST
- Food type: TETRAMIN (commercial food).
- Amount: not specified
- Frequency: the fish are fed daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Remarks on exposure duration:
At different reading times, mortalities are recorded.
Hardness:
150 mg/L CaCO3
Test temperature:
22 deg C
pH:
pH 7.8-8
Dissolved oxygen:
no data
Salinity:
no data
Conductivity:
no data
Nominal and measured concentrations:
Concentration:
35000-100000 ug/L for 2 and 4 hours
10000-35000 ug/L for 6 and 24 hours
Details on test conditions:
TEST SYSTEM
- Test vessel: The aquariums are covered with a glass plate during the tests
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: aquariums with a capacity of 7 liters
- Aeration: no aeration is practiced
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): not specified
- No. of organisms per vessel: Five fishes were used for each concentration.
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified
- No. of vessels per vehicle control (replicates): not specified
- Biomass loading rate: not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fish toxicity tests are carried out in aquariums with a capacity of 7 liters containing 2 liters of test solution. - Total organic carbon: not specified
- Particulate matter: not specified
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Culture medium different from test medium: not specified
- Intervals of water quality measurement: not specified

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: the fihes were placed in controlled room under a natural lighting.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not specified
- Justification for using less concentrations than requested by guideline: not specified
- Range finding study not specified
- Test concentrations: < 1 mg/I, 1-3,5; 3,5-10; 10-35; 35-100 and >= 100 mg/l
- Results used to determine the conditions for the definitive study: AFNOR T 90-303 (1978)


Reference substance (positive control):
yes
Duration:
2 h
Dose descriptor:
LC50
Effect conc.:
> 35 000 - < 100 000 µg/L
Nominal / measured:
not specified
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
4 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 - < 35 000 µg/L
Nominal / measured:
not specified
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
6 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 - < 35 000 µg/L
Nominal / measured:
not specified
Conc. based on:
act. ingr.
Basis for effect:
mortality
Key result
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 - < 35 000 µg/L
Nominal / measured:
not specified
Conc. based on:
act. ingr.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
The LC 50 (24h) was in the range 10-35 mg/l  for Brachydanio reri, at test described test conditions.
Considering the results, accoring to CLP classification critiria, the substance was judged as hazardous to the aquatic environment: the substance has an harmonized classification as Aquatic Chronic 3 H412
Executive summary:

The acute toxicity of analytical grade of Pyrogallol has been tested on Brachydanio rerio after different periods of contact: 2, 4, 6 and 24 hours. The LC50 were determined.

Description of key information

The acute toxicity of Pyrogallol was tested on Brachydanio rerio and observed at 2, 4, 6 and 24 hours, according to ISO/DIS 7346/1; the preliminary test was performed according to AFNOR T 90-303 (1978).

Key value for chemical safety assessment

LC50 for freshwater fish:
10 mg/L

Additional information

The LC 50 (24h) was in the range 10-35 mg/l  for Brachydanio rerio, at test described test conditions.

According to the results, the substance was considered as hazardous for aquatic environment and classified as Aquatic chronic 3 H412.