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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Evaluation of a Three-Exposure Mouse Bone Marrow Micronucleus Protocol: Results with 49 Chemicals
Author:
M.D. Shelby, G.L. Erexson, G.J. Hook and R.R. Tice
Year:
1993
Bibliographic source:
Environmental and Molecular Mutagenesis 21: 160-179 (1993)

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
Deviations:
yes
Remarks:
Only a single sex was used.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Test animals

Species:
mouse
Strain:
B6C3F1
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
Each chemical was prepared in the appropriate solvent (PBS for water soluble chemicals, corn oil for water-insoluble chemicals).
Duration of treatment / exposure:
Three consecutive day
Frequency of treatment:
Daily
Post exposure period:
48 hr after the third treatment, the surviving mice were euthanized
Doses / concentrationsopen allclose all
Dose / conc.:
37.5 mg/kg bw/day (nominal)
Dose / conc.:
75 mg/kg bw/day (nominal)
Dose / conc.:
150 mg/kg bw/day (nominal)
No. of animals per sex per dose:
Groups of 5 mice were administered the test chemicals.
Control animals:
yes

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls valid:
yes

Applicant's summary and conclusion

Conclusions:
AITC did not increase the number of micronuclei in bone marrow cells.