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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity of Isothiocyanates, Isocyanate and Thioureas on Salmonella Typhimurium
Author:
Tsutomu Yamaguchi
Year:
1980
Bibliographic source:
Agric. Biol. Chem.,44 (12), 3017-3018, 1980

Materials and methods

Principles of method if other than guideline:
The mutagenicity of isothiocyanates and related compounds was tested by the method of Ames et al. with some modifications.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Method

Species / strainopen allclose all
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Metabolic activation system:
S-9 Mix
Species / strain:
other: TA1535, TA1537,TA1538
Metabolic activation:
not specified
Vehicle:
DMSO

Results and discussion

Test resultsopen allclose all
Species / strain:
other: TA100, TA98
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity:
not specified
Vehicle controls valid:
not specified
Negative controls valid:
not specified
Positive controls valid:
not specified
Species / strain:
other: TA1535, TA1537, TA1538
Metabolic activation:
not specified
Genotoxicity:
negative
Cytotoxicity:
not specified
Vehicle controls valid:
not specified
Negative controls valid:
not specified
Positive controls valid:
not specified
Additional information on results:
There was no activating effect on mutagenicity by the pretreatment of these samples with S-9 Mix.

Applicant's summary and conclusion

Conclusions:
It was found that all of the isohiocyanates tested showed mutagenicity on Salmonella typhimurium TA100, though the degree varied with each sample. Especially, allyl isothiocyanate have the highest potency among these compunds.