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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Antimicrobial Properties of Sinigrin and its Hydrolysis Products
Author:
B.G. Shofran, S.T. Purrngton, F. Breidt and H.P.Fleming
Year:
1998
Bibliographic source:
Volume 63, No. 4, 1998 - JURNAL OF FOOD SCIENCE

Materials and methods

Principles of method if other than guideline:
Allyl isothiocyanate was tested for its Minimum Inhibitory Concentration (MIC) in broth to 9 species of bacteria and 8 species of yeasts as described by Kyung and Fleming (1996).
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Test solutions

Vehicle:
not specified

Study design

Test type:
not specified
Total exposure duration:
48 h
Remarks on exposure duration:
Incubation continued beyond 48h to 96h to observe any changes in those compunds that caused no visible turbidity.

Test conditions

Test temperature:
30° C or 37° C.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
other: Minimum Inhibitory Concentration (MIC)
Remarks:
Bacteria
Effect conc.:
> 50 - < 1 000 other: ppm
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
growth inhibition
Remarks on result:
other: Bacteria
Duration:
48 h
Dose descriptor:
other: Minimum Inhibitory Concentration (MIC)
Remarks:
Nonxerotolerant Yeasts
Effect conc.:
> 1 - < 4 other: ppm
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
growth inhibition
Remarks on result:
other: Nonxerotolerant Yeasts
Duration:
48 h
Dose descriptor:
other: Minimum Inhibitory Concentration (MIC)
Remarks:
Xerotolerant Yeasts
Effect conc.:
ca. 50 other: ppm
Nominal / measured:
not specified
Conc. based on:
not specified
Remarks on result:
other: Xerotolerant Yeasts: it retarded but did not prevent growth.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Results indicated that AITC was the most inhibitory of the sinigrin hydrolysis products. AITC had an MIC of 50 to 1000 ppm for bacteria and 1 to 4 ppm for nonxerotolerant yeasts, and, against xerotolerant yeasts at 50 ppm, it retarded but did not prevent growth.