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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Cross-reference
Reason / purpose:
reference to same study
Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
See read-across justification attached in section 13.2
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Test material information:
Composition 1
Analytical monitoring:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Origin: Strain of Bundesgesundheitsamt Berlin
- Maintenance and acclimatisation:
- A population of parthenogenetic females of synchronized age structure is maintained under constant temperature conditions (20 +/- 1°C) at a
16:8 hour light-dark photoperiod (illumination: < 1000 lux).
- M4 medium is partly renewed once a week
- Daphnia are exclusively fed with unicellular green algae ad libitum
- The neonates are separated from their parent Daphnia by filtration prior to the acute test
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.1 °C
pH:
7.7
Dissolved oxygen:
8.4 mg/L
Nominal and measured concentrations:
Test concentrations (mg/L): 3.4, 6.9, 13.8, 27.5 and 55 (nominal)
Details on test conditions:
- Test vessels: 50 ml glass beakers holding 10 meonates in 20 ml of test medium
- Experimental design:
- 5 test concentrations plus 1 control
- 10 neonates per vessel, 2 replicates per concentration / control
- no feeding during the exposure period
- Photoperiod: 16 h light : 8 h dark
- Aeration: none
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.6 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
48 h EC0 1.3 mg/L (measured)
48 h EC100 9.8 mg/L (measured)
Results with reference substance (positive control):
not specified
Validity criteria fulfilled:
not specified
Conclusions:
According to the study carried out on the mobility of Daphnids, the EC50 of the test substance was found to be 3.6 mg/L (based on the measured geometrical mean).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Origin: Strain of Bundesgesundheitsamt Berlin
- Maintenance and acclimatisation:
- A population of parthenogenetic females of synchronized age structure is maintained under constant temperature conditions (20 +/- 1°C) at a
16:8 hour light-dark photoperiod (illumination: < 1000 lux).
- M4 medium is partly renewed once a week
- Daphnia are exclusively fed with unicellular green algae ad libitum
- The neonates are separated from their parent Daphnia by filtration prior to the acute test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.1 °C
pH:
7.7
Dissolved oxygen:
8.4 mg/L
Nominal and measured concentrations:
Test concentrations (mg/L): 3.4, 6.9, 13.8, 27.5 and 55 (nominal)
Details on test conditions:
- Test vessels: 50 ml glass beakers holding 10 meonates in 20 ml of test medium
- Experimental design:
- 5 test concentrations plus 1 control
- 10 neonates per vessel, 2 replicates per concentration / control
- no feeding during the exposure period
- Photoperiod: 16 h light : 8 h dark
- Aeration: none
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.6 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
48 h EC0 1.3 mg/L (measured)
48 h EC100 9.8 mg/L (measured)
Results with reference substance (positive control):
not specified

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
According to the study carried out on the mobility of Daphnids, the EC50 of the test substance was found to be 3.6 mg/L (based on the measured geometrical mean).