Condensation products of fatty acids, tall oil with 2-amino-2-ethylpropanediol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

28 March 1991-5 July 1991

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Specific details on test material used for the study:

Test Material Name: Alkaterge E
Chemical name: 2-heptadecenyl-4-ethyl-2-oxazolin-4-methanol
CAS Number: 68140-98-7
Batch nr.: 1-59
Appearance: Liquid, brown, viscous
Molecular weight: 365.60 g/mol

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
The test substance is not soluble in water and remains at the surface in aqueous solutions. The test substance was directly transferred to the test vessels to which 10 ml growth medium and 10 ml of the bacterial suspension were added. Distilled water was added to a final volume of 100 ml.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas putida from the German collection of microorganisms
- Method of cultivation: pre-culture of the bacteria for 7 hours at 21 º C
- Preparation of inoculum for exposure: the test substance was directly transferred to the test vessels to which 10 ml growth medium and 10 ml of the bacterial suspension were added. Distilled water was added to a final volume of 100 ml.
:

Test type:

static

Water media type:

other: distilled water

Limit test:

no

Total exposure duration:

16 h

Hardness:

no data

Test temperature:

21 º C

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

Nominal concentrations: 5, 8 and 10 g/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- No. of vessels per abiotic control (replicates):
- Sludge concentration (weight of dry solids per volume):
- Weight of dry solids per volume of reaction mixture per unit of time:
- Nutrients provided for bacteria:
- Nitrification inhibitor used (delete if not applicable): none / N-allylthiourea
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Particulate matter:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
- Details on termination of incubation:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using fewer concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

not required

Key result

Duration:

16 h

Dose descriptor:

other: 10 % growth increase

Effect conc.:

5 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth increase

Key result

Duration:

16 h

Dose descriptor:

other: 2% growth increase

Effect conc.:

8 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth increase

Key result

Duration:

16 h

Dose descriptor:

other: 4% growth increase

Effect conc.:

10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth increase

Details on results:

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:
- Adsorption (e.g. of test material to the walls of the test container):
- Blank controls oxygen uptake rate:
- Coefficient of variation of oxygen uptake rate in control replicates:

Validity criteria fulfilled:

not applicable

Conclusions:

Alkaterge E does not show any growth inhibition in the test with Pseudomonas putida.

Description of key information

The available acute toxicity study in microorganisms (Pseudomonas putida) is considered to be invalid due to the use of concentrations which are above the water solubility.