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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: domestic STP east of Suzhou city (no. 20150731)
- Preparation of inoculum for exposure: sedimented sludge was resuspended in test medium 3 times
- Pretreatment: none
- Concentration of sludge: 4.0 g suspended solid/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 60 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: buffer solution with Ca, Mg, and Fe added
- Test temperature: 20+-1 °C
- pH: 7.49
- Aeration of dilution water:
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Number of culture flasks/concentration: 2
SAMPLING
- Sampling frequency: weekly
- Sampling method: BOD analyzer
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 22.4
- Sampling time:
- 18 d
- Remarks on result:
- other: 10-d time window began between D8 (9.4%) and D9 (11.2% degr.)
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 21.6
- Sampling time:
- 19 d
- Results with reference substance:
- 83.5% degradation on D14
- Validity criteria fulfilled:
- yes
- Remarks:
- 1) Difference between replicates was 2.9% (<20%) 2) Ref.item was degraded by 83.5% in 14 days ( min.60%) 3) Avg. O2 uptake of inoculum blank was 21.2 mg/L in 28 days (max. 60 mg/L) 4) Toxicity control showed 52.1% degradation on D14 (min. 25%)
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Di-PETMP is not readily biodegradable, but can be assumed to be inherently biodegradable with > 20% biodegradation.
Reference
Description of key information
Based on 62.2% biodegradation in a closed bottle test and the 10 d window criterion being fulfilled, BuMP is readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
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