Sodium tetrafluoroborate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium tetrafluoroborate

EC Number:

237-340-6

EC Name:

Sodium tetrafluoroborate

Cas Number:

13755-29-8

Molecular formula:

NaBF4

IUPAC Name:

sodium tetrafluoroborate

Details on test material:

- Name of test material (as cited in study report): NaBF4
- Molecular formula (if other than submission substance): NaBF4
- Molecular weight (if other than submission substance): 109.79 g/mol
- Smiles notation (if other than submission substance): [B-](F)(F)(F)F.[Na+]
- InChl (if other than submission substance): InChI=1/BF4.Na/c2-1(3,4)5;/q-1;+1
- Structural formula attached as image file (if other than submission substance): see Fig.

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

0.15 mmol/L (= 15 mg/L as CaCO3)

Test temperature:

23ºC

pH:

7.5

Nominal and measured concentrations:

Nominal test concentrations for the main test were chosen: 6.25, 12.5, 25, 50 and 100 mg/L

Details on test conditions:

75 mL Erlenmeyer flasks were used as incubation vessels. The volume of test solution in each test flask was 30 mL per replicate. Each test flask was covered with a glass dish. The test flasks were labeled with the study number and all necessary additional information to ensure unique identification.
The test design included three replicates per test concentration and six replicates of the control.

The test was started using a nominal algal cell density of 5000 cells/mL. The initial cell density was selected according to the recommendations of the OECD test guideline. The algal cell density in the pre-culture was determined using an electronic particle counter (Cell Counter CASY TT, OLS, Bremen/Germany). A static test design was applied. The duration of the test was 72 hours.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 72-hour NOEC for growth rate and yield was determined to be 50 mg/L, since up to and including this concentration the growth rate and yield of the algae after 72 hours were not significantly lower than in the control. The LOEC for growth rate and yield was determined to be 100 mg/L.