Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Tolerance of Chlorella vulgaris for metallic and non-metallic ions
Author:
den Dooren de Jong, L.E. & Roman, W.B.
Year:
1965
Bibliographic source:
Antonie an Leeuwenhoek 31: 301-313; https://doi.org/10.1007/BF02045910

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
No guideline mentioned: systematic investigation of the tolerance of C. vulgaris for both metallic (49) and non-metallic (7) ions.
GLP compliance:
no

Test material

Reference
Name:
Unnamed

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Chlorella vulgaris
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): Laboratory of Microbiology, Techn. University Delft, The Netherlands


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
Several months - not reported exactly.

Test conditions

Hardness:
no data
Test temperature:
room temperature
pH:
ca. 7
Dissolved oxygen:
no data
Nominal and measured concentrations:
0 (control) and a range of 0.0001 - 30% of the metal salt.
Details on test conditions:
TEST SYSTEM
- Test vessel: 150 mL Erlenmeyer vessels
- Fill volume: 50 mL
- Type (open/closed): flasks were stoppered with cotton plugs
- Initial cells density: not measured, for inoculation, cells were taken from Chlorella cultures on agar slants. The amount was kept so small that no green tinge could be observed after shaking the inoculated medium.

GROWTH MEDIUM
- Standard medium used: no
- Detailed composition if non-standard medium was used: The basal medium consisted of: 0.05% K2HPO4; 0.05% KH2PO4; 0.05% (NH4)2SO4; 0.05% KNO3; copper-free distilled water, 20% of which was replaced by activated-carbon-treated tapwater or well-water (salts used were of analytical grade).


OTHER TEST CONDITIONS
- Sterility: medium was sterilized by heating at 110 °C for 20 min
- Photoperiod, light intensity and quality: daylight

EFFECT PARAMETERS MEASURED:
- Effects were observed contineously. The highest concentration tolelaretd by the organisms as well as the lowest concentration inhibiting growth were recorded.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Dose descriptor:
NOEC
Effect conc.:
3.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Remarks on result:
other: duration: not specified
Dose descriptor:
LOEC
Effect conc.:
7.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Remarks on result:
other: duration: not specified

Any other information on results incl. tables

Since photoperiod was not continuous, growth rate was low and first differences to the blank cultures were observed some weeks after test start. Observations were made for 3 - 4 months.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable