2-ketogluconic acid, calcium salt

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-ketogluconic acid, calcium salt

EC Number:

919-553-2

Cas Number:

28098-92-2

Molecular formula:

C6H10O7.xCa

IUPAC Name:

2-ketogluconic acid, calcium salt

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Remarks:

HPLC

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Remarks:

ASTM medium-hard medium

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Total Hardness betwee 165 - 184 mg/L as CaCO3

Test temperature:

Temperature range during exposure : 20.5 - 23.0 °C

pH:

pH range during exposure : 8.1 - 8.4

Dissolved oxygen:

Dissolved Oxygen range during erxposure : 7.5 - 7.8 mg/L

Details on test conditions:

Exposure vessel : 100 mL glass crystallising dish
Duplicates : 2
No. of Neonates per Vessel : 10

Temperature : 20.5 - 23 °C
Light : Normal laboratory light intensity
Light / Dark Periods : 16 hour light / 8 hour dark

The tes solutions were prepared the day before the neonates were exposed to them. At the start of the test the Daphnids were added to the test vessels and watchglasses were placed over the test vessels to isolate the Daphnids and also to prevent them from coming into contact with the air-water interface.This avoids possible indirect effects from being caught in any surface film which might have developed. During the addition of the Daphnids, care was taken not to disturb the test medium. The exposure vessels were placed in the test area according to a random distribution.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 (48 hour) = 17 g/L (95% Confidence Limits : 0.4 - 0.5 mg/L)
EC0 (48 hour) = 14 g/L
EC90 (48 hour) = 20 g/L