Sodium 3-sulphonatopropyl acrylate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 Aug - 17 Aug 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

The Department of Health of the Government of the United Kingdom

Test material

Test material information

Constituent 1

Reference substance name:

Potassium 3-sulphonatopropyl acrylate

EC Number:

250-465-0

EC Name:

Potassium 3-sulphonatopropyl acrylate

Cas Number:

31098-20-1

Molecular formula:

C6H10O5S.K

IUPAC Name:

potassium 3-(acryloyloxy)propane-1-sulfonate

Details on test material:

- Name of test material (as cited in study report): SPA (sulfopropyl acrylate)
- Physical state: powder
- Analytical purity: no data
- Lot/batch No.: 0200014/001
- Storage condition of test material: at room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Samples were taken at 0 h and after each test media renewal at 24, 48 and 72 h, representing those of the freshly prepared and old test preparations.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 4 g test substance were dissolved in dechlorinated tap water and treated with ultrasonication for approx. 2 min. The volume was adjusted to 1 L to give a 4 g/L stock solution which was inverted several times to ensure adequate mixing and homogeneity. An aliquot of 500 mL stock solution was dispersed into the final volume of 20 L resulting in a concentration of 100 mg/L.
- Evidence of undissolved material: The test preparations were observed to be clear, colourless solutions throughout the study.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: in-house culture since June 2001, originally obtained from Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, United Kingdom
- Age at study initiation: juvenile
- Length at study initiation: mean: 4.8 cm (SD = 0.6)
- Weight at study initiation: mean: 1.51 g (SD = 0.32)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 1 Aug - 13 Aug 2001
- Acclimation conditions: same as test
- Type and amount of food: commercial trout pellets
- Health during acclimation: < 1% mortality 7 days prior to test start

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

100 mg CaCO3/L

Test temperature:

14 °C, measured at test start and after 24, 48, 72 and 96 h in every test vessel and the control.

pH:

7.4 - 7.7, measured at test start and after 24, 48, 72 and 96 h in every test vessel and the control.

Dissolved oxygen:

9.0 - 9.6 mg O2/L (84 - 95% of saturation) measured at test start and after 24, 48, 72 and 96 h in every test vessel and the control.

Nominal and measured concentrations:

nominal: 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel
- Type: Test vessels were covered with glass lids to reduce evaporation.
- Material, fill volume: glass tanks, containing 20 L test solution
- Aeration: yes, via narrow bore glass tubes
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.76 g bw/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water was dechlorinated by passage through an activated carbon filter (Purite Series 500) and partly softened (Elga Nimbus 1248D Duplex Water Softener).

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark with 20 min dawn and dusk transition periods

EFFECT PARAMETERS MEASURED: mortality and sub-lethal effects after 3, 6, 24, 48, 72 and 96 h.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Neither mortality nor sub-lethal effects were observed up to a concentration of 100 mg/L.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No sub-lethal effects were observed.
- Mortality of control: no

Any other information on results incl. tables

Sublethal observations / clinical signs:

Analysis of the test preparations sampled at test start, after 24 h and after 96 h showed the measured test concentrations to be near nominal (97 - 104%).