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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the category approach justification provided in section 13.
Reason / purpose:
read-across source
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
51 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
mobility
Remarks on result:
other:
Remarks:
Source, key, 52292-17-8, BASF, 2000, R0000576, D. magna, 48 h, RL2
Conclusions:
The key effect value for short-term toxicity to aquatic invertebrates is an EL50 (48 h) of 51 mg/L (nominal, OECD 202).
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 - 25 Nov 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: waterflea
- Source: originally obtained from BioInternational B.V., NJ Horn, Netherlands
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
21 °C
pH:
7.9 - 8.0
Dissolved oxygen:
97% - 98%
Nominal and measured concentrations:
nominal (WAF): 0, 1, 3, 10, 30 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL glass beakers, covered with glass plates. Test volume: 50 mL
- Renewal rate of test solution (frequency/flow rate): no renewal as static test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium according to Elendt
- Intervals of water quality measurement: test start and test end


OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h darkness
- Light intensity: approx. 900 lux

Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
51 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50 (24 h): 0.9 mg/L (within the range of 0.9 and 1.9 mg/L)

Nominal concentration
[mg/L]

Test period (h)

24 h

48 h

Immobilized daphnids

Immobilized daphnids

0

0

0

 

0

1

1

0

2

 

0

1

3

0

3

 

0

2

10

0

1

 

0

2

30

1

2

 

0

3

100

2

8

 

2

8

Description of key information

Crustacea: EL50 (48h) = 51 mg/L for Daphnia magna

Key value for chemical safety assessment

Additional information

As there are no data available for alcohol ethoxylate C16, <2.5EO (CAS 9004-95-9) the acute toxicity to aquatic invertebrates is assessed on the basis of data from a study with a similar alcohol ethoxylate (iso-stearyl-ethoxylate 2EO, CAS 52292-17-8). The test was conducted according to OECD 202 and GLP, with Daphnia magna as test organism. Due to the very low water solubility of the substance, the test media were prepared as Water Accommodated Fraction (WAF). In the study an EL50 of 51 mg/L was determined (BASF, 2000).