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EC number: 947-957-9 | CAS number: -
Acute oral toxicity was assessed in:
1) an OECD 423 study for a read-across substance, Phosphoric acid, 2-ethylhexl ester, the LD50 was 2500 mg/kg bw - Category 5 acute toxicity (GHS);
2) an OECD 401 (equivalent study) for a read-across substance, phosphoric acid ester (linear C8), the LD50was greater than 2000 mg/kg bw - Category 5 acute toxicity (GHS).
Acute inhalation and dermal toxicity studies were waived on the basis of skin corrosivity.
Table 1: Mortality Data
Dose Level mg/kg
Number of Animals Treated
Deaths During Day of Dosing(Hours)
Deaths During Period After Dosing(Days)
Table 2: Individual Clinical Observations - 300 mg/kg
Animal Number and Sex
Effects Noted After Dosing(Hours)
Effects Noted During Period After Dosing(Days)
0= No signs of systemic toxicity
Table 3: Individual Clinical Observations - 2000 mg/kg
0= No signs of systemic toxicity
Rn = Noisy respiration
S = Increased salivation
Ss = Red/brown staining around the snout
X = Animal dead
Table 4: Individual Bodyweights and Weekly Bodyweight Changes - 300 mg/kg
Animal Numberand Sex
Bodyweight (g) at Day
Bodyweight Gain (g) During Week
Table 5: Individual Bodyweights and Weekly Bodyweight Changes - 2000 mg/kg
Bodyweight (g) at Death
Table 6: Individual Necropsy Findings - 300 mg/kg
Time of Death
Killed Day 14
No abnormalities detected
Table 7: Individual Necropsy Findings - 2000 mg/kg
Found Dead Day 2
Liver: patchy pallor
Gastric mucosa: haemorrhagic
The study was performed to assess the acute oral toxicity of the test item following a single oral
administration in the Wistar strain rat. The method was designed to be compatible with the following:
- OECD Guidelines for the Testing of Chemicals No. 423 “Acute Oral Toxicity – Acute Toxic Class
Method” (adopted 17 December 2001) - Method B1 tris Acute Toxicity (Oral) of CommissionRegulation (EC) No. 440/2008
A group of three fasted females was treated with the test item at a dose level of 300 mg/kg bodyweight. Based on the results from this dose level further groups of fasted females were treated at
a dose level 2000 mg/kg bodyweight. Dosing was performed sequentially. The test item was administered orally as a solution in distilled water. Clinical signs and bodyweight development were monitored during the study. All animals were subjected to gross necropsy.
One animal treated at a dose level of 2000 mg/kg was found dead two days after dosing.
Signs of systemic toxicity noted in one animal treated at a dose level of 2000 mg/kg were increased salivation, red/brown staining around the snout and noisy respiration. There were no other signs of systemic toxicity noted.
The surviving animals showed expected gains in bodyweight over the study period.
Abnormalities noted at necropsy of the animal that died during the study were patchy pallor of the liver,
dark spleen, dark kidneys and haemorrhage of the gastric mucosa. No other abnormalities were noted
at necropsy of animals that were killed at the end of the study.
The acute oral median lethal dose (LD50) of the test item in the female Wistar strain rat was
approximately 2500 mg/kg bodyweight (Globally Harmonised Classification System ‑ Category 5,
>2000 ‑ 5000 mg/kg bodyweight).
Five male Fisher 344 rats were orally dosed with 2000 mg/kg bw of Hordaphos MDO (phosphoric acid ester - linear C8). The animals were allowed free access to food and water over an observation period of seven days. There were no treatment related deaths. The LD50 was determined to be greater than 2000 mg/kg bw.
The acute oral median lethal dose (LD50) of the test item in the female Wistar strain rat was approximately 2500 mg/kg bodyweight (Globally Harmonised Classification System - Category 5, >2000 -5000 mg/kg bodyweight).
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