Silver methanesulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study, published in peer reviewed literature, some limitations in design and/or reporting but otherwise adequate for assessment.

Data source

Reference

Reference Type:

publication

Title:

Evaluation of the effect of reactive sulfide on the acute toxicity of silver (I) to Daphnia magna, part 2: Toxicity results.

Author:

Bianchini A, Bowles KC, Brauner CJ, Gorsuch JW, Kramer JR and Wood CM

Year:

2002

Bibliographic source:

Environmental Toxicology and Chemistry 21:1294-1300

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

48-hour static renewal toxicity tests. Acute AgNO3 toxicity was tested at two different sulphide concentrations (mean ± standard deviation): 250 ± 10 nM and 22 ± 0.4 nM.

GLP compliance:

not specified

Test material

Test material information

impurity 1

Reference substance name:

Silver methanesulphonate

EC Number:

219-199-2

EC Name:

Silver methanesulphonate

Cas Number:

2386-52-9

Molecular formula:

CH4O3S.Ag

IUPAC Name:

silver methanesulphonate

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Full details provided in partner publication. Bowles et al. ET&C Vol. 21

Test solutions

Vehicle:

no

Details on test solutions:

In the absence of sulfide, nominal concentrations were 0 (control), 0.05, 0.1, 0.2, 0.5, 1, and 2 µg Ag/L (0, 0.46, 0.93, 1.9, 4.6, 9.3, and 19 nM) in both toxicity tests

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Source aquatic Research Organisms, Hampton, NH, USA (ARO strain, lot 090600 brood origination USEPA, Cincinnati, OH, USA). Acclimated to synthetic water used in tests; fed a slurry of yeast, cerophyll, and trout chow. Following acclimation, reproduction rate was measured to ensure it met established criteria for a healthy population. Neonates passed through the mesh and into the aquarium of synthetic water and were collected within 6 to 24h of hatch for toxicological studies.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Also 24h

Test conditions

Hardness:

115mg/L CaCO3

Test temperature:

20

pH:

8,2

Dissolved oxygen:

PO2 = 164.2 ± 1.9 mmHg; n = 10

Salinity:

not applicable

Nominal and measured concentrations:

0, 0.5, 0.1, 0.2, 0.5, 1 and 2 µg Ag/L (nominal) in the absence of sulfide

Details on test conditions:

Synthetic water in borosilicate glass beakers, acid washed in 1% HNO3 and rinsed with synthetic water prior to use. Three replicas of ten organisms per vessel were used with no aeration. Solution renewed after 24 hours. A 16 hour light, 8 hour dark day was used. The organisms were not fed.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.22 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

element (dissolved fraction)

Basis for effect:

mortality

Remarks on result:

other: 95% CL 0.19–0.25 µg Ag /L. Filtered (filtered, 0.45-mm), silver concentration.

Details on results:

results also for 24 hour LC50 and with added sulphide

Any other information on results incl. tables

Reactive sulfide protects D magna against acute silver toxicity.

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The freshwater 48hr LC50 values for daphnia magna when exposed to silver nitrate are 0.18 to 0.26 µg Ag/L based on measured total silver concentration and 0.22 µg Ag/L based on measured filtered silver concentration.

Executive summary:

The study is a non-guideline study, published in peer reviewed literature and considered suitable for use as a key study for this endpoint. The freshwater 48hr LC50 values for daphnia magna when exposed to silver nitrate are 0.18 to 0.26 µg Ag/L based on measured total silver concentration and 0.22 µg Ag/L based on measured filtered silver concentration.