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EC number: 219-199-2 | CAS number: 2386-52-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non-guideline study, published in peer reviewed literature, some limitations in design and/or reporting but otherwise adequate for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the effect of reactive sulfide on the acute toxicity of silver (I) to Daphnia magna, part 2: Toxicity results.
- Author:
- Bianchini A, Bowles KC, Brauner CJ, Gorsuch JW, Kramer JR and Wood CM
- Year:
- 2 002
- Bibliographic source:
- Environmental Toxicology and Chemistry 21:1294-1300
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- 48-hour static renewal toxicity tests. Acute AgNO3 toxicity was tested at two different sulphide concentrations (mean ± standard deviation): 250 ± 10 nM and 22 ± 0.4 nM.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Silver methanesulphonate
- EC Number:
- 219-199-2
- EC Name:
- Silver methanesulphonate
- Cas Number:
- 2386-52-9
- Molecular formula:
- CH4O3S.Ag
- IUPAC Name:
- silver methanesulphonate
- Test material form:
- solid
impurity 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Full details provided in partner publication. Bowles et al. ET&C Vol. 21
Test solutions
- Vehicle:
- no
- Details on test solutions:
- In the absence of sulfide, nominal concentrations were 0 (control), 0.05, 0.1, 0.2, 0.5, 1, and 2 µg Ag/L (0, 0.46, 0.93, 1.9, 4.6, 9.3, and 19 nM) in both toxicity tests
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Source aquatic Research Organisms, Hampton, NH, USA (ARO strain, lot 090600 brood origination USEPA, Cincinnati, OH, USA). Acclimated to synthetic water used in tests; fed a slurry of yeast, cerophyll, and trout chow. Following acclimation, reproduction rate was measured to ensure it met established criteria for a healthy population. Neonates passed through the mesh and into the aquarium of synthetic water and were collected within 6 to 24h of hatch for toxicological studies.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Also 24h
Test conditions
- Hardness:
- 115mg/L CaCO3
- Test temperature:
- 20
- pH:
- 8,2
- Dissolved oxygen:
- PO2 = 164.2 ± 1.9 mmHg; n = 10
- Salinity:
- not applicable
- Nominal and measured concentrations:
- 0, 0.5, 0.1, 0.2, 0.5, 1 and 2 µg Ag/L (nominal) in the absence of sulfide
- Details on test conditions:
- Synthetic water in borosilicate glass beakers, acid washed in 1% HNO3 and rinsed with synthetic water prior to use. Three replicas of ten organisms per vessel were used with no aeration. Solution renewed after 24 hours. A 16 hour light, 8 hour dark day was used. The organisms were not fed.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.22 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- element (dissolved fraction)
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL 0.19–0.25 µg Ag /L. Filtered (filtered, 0.45-mm), silver concentration.
- Details on results:
- results also for 24 hour LC50 and with added sulphide
Any other information on results incl. tables
Reactive sulfide protects D magna against acute silver toxicity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The freshwater 48hr LC50 values for daphnia magna when exposed to silver nitrate are 0.18 to 0.26 µg Ag/L based on measured total silver concentration and 0.22 µg Ag/L based on measured filtered silver concentration.
- Executive summary:
The study is a non-guideline study, published in peer reviewed literature and considered suitable for use as a key study for this endpoint. The freshwater 48hr LC50 values for daphnia magna when exposed to silver nitrate are 0.18 to 0.26 µg Ag/L based on measured total silver concentration and 0.22 µg Ag/L based on measured filtered silver concentration.
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