Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-917-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The limit concentration and the control were analytically verified from freshly prepared media at after 0 and 72 h and from the corresponding 24 h aged test media after 24 and 96 h.
All original samples were stored at room temperature before preparation. Prepared samples were stored in an autosampler at room temperature until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was freshly prepared in demineralized tap water. The dispersion was agitated until the stock solution was clear. After transferring an appropriate volume of the stock solution into the test vessel (filled with river water), the test medium was mixed with an ultraturrax (1 min, at least 17000 rpm). This procedure was repeated daily for the preparation of the stock solution and the water renewal of the threshold concentration. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: All fish used in the test was gained at Noack Laboratorien GmbH from a single brood stock (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin)
- Length at study initiation (length definition, mean, range): 2.3 cm (2.0 – 2.5 cm)
- Weight at study initiation (mean): 0.11 g
ACCLIMATION
- Acclimation period: 7 d
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: Food (Sera Vipan; SERA GMBH, 52518 Heinsberg, Germany) was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day.
- Health during acclimation (any mortality observed): No mortality was observed. No disease treatments were administered throughout holding and testing.
FEEDING DURING TEST
- The test fish were not fed 24 h before test start. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 67 mg/L as CaCO3
- Test temperature:
- 22.3 - 22.8
- pH:
- 7.15 - 7.64
- Dissolved oxygen:
- saturation 96-99%
- Nominal and measured concentrations:
- 146 µg/L, corresponding to 38.8 µg a.i./L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 3 L, filled with about 2.5 L of natural river water (dimensions: 11.5/13/20 cm, depth of water: about 16.5 cm), and covered with glass plates.
- Aeration: yes
- Renewal rate of test solution: daily renewal
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.30 g fish per litre test solution
TEST MEDIUM / WATER PARAMETERS
- see Any other information on material and methods
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 7 – 750 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Observations were made after 3, 24, 48, 72 and 96 h
Fish were considered dead if there was no visible movement (e.g. gill covers movement) and if touching of the caudal peduncle produced no reaction. Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behavior, respiratory function, pigmentation, etc.).
TEST CONCENTRATIONS
- limit test; selection of the test concentration was based on the derivation of a threshold concentration (TC) from the results of an alga toxicity test (ErC50 (0-72 h) = 146 µg/L (nominal test item concentration) and a daphnia acute immobilization test
(EC50 (0-48 h) = 609 µg/L (nominal test item concentration)) - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 38.8 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 146 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none observed
- Mortality of control: no mortality observed in the control - Sublethal observations / clinical signs:
Validity criteria:
· The O2 saturation should be >/=60% (this study: >/=96%).
· The mortality in the control group should not exceed 1/7 fish (this study: 0/7 fish).
· The pH-value in the control group should not deviate by more than one unit over the test period (this study: 0.47 units).
Observations in the Definitive Test
(n = 7)
Nominal dry content concentration of the test item
[µg/L]Observation*)
Number of fish effected
after different exposure periods [hours]3
24
48
72
96
38.8
(1)
7
7
7
7
7
Control
(1)
7
7
7
7
7
*)The number in brackets corresponds to the following observation:
(1) = Normal behavior
Cumulative Mortality [%] in the Definitive Test
Nominal dry content concentration of the test item
[µg/L]Cumulative Mortality [%] after different exposure periods [hours]
3
24
48
72
96
38.8
0
0
0
0
0
Control
0
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC0 after 96 hours was ≥ 146 µg test item/L, corresponding to ≥ 38.8 µg/L of the dry content of the test item.
- Executive summary:
The acute toxicity of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid (26.6% a.i.) to fish (zebrafish) was determined according to OECD Guideline 203 (1992) and EU Method C.1 (2008). An acute toxicity test under semi-static conditions with natural river water and daily renewal of the test media was conducted with the limit concentration of 146 µg/L of the test item, corresponding to a nominal dry content concentration of 38.8 µg/L. Duration of the test was 96 hours. Seven test organisms were exposed to the limit concentration and the control. Water quality parameters (temperature, pH-value and oxygen-saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits.
The limit concentration of the test item and the control were analytically verified via LC-MS/MS from freshly prepared media after 0 and 72 h and from corresponding 24 h aged test media after 24 and 96 h. The measured concentrations at the start of the exposure intervals after 0 and 72 hours were 94 and 100% of the nominal value. At the end the exposure intervals after 24 to 96 hours the measured test item concentrations were 41and 39% of the nominal value.
Since the test is an UVCB substance all effect values given are based on the nominal concentration of the test item and its dry content. All validity criteria of the test guideline were met.
The LC0 was >/=146 µg/L in terms of test material, corresponding to >/=38.8 µg a.i./L.
Results Synopsis
Test organism size/age: Danio rerio (mean length: 2.3 cm; mean weight: 0.11 g)
Test Type: semi-static
LC50: >/= 38.8 µg a.i./L
Endpoint(s) Effected: mortality and sublethal effects
Reference
Description of key information
LC0 >/= 38.8 µg a.i./L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 38.8 µg/L
Additional information
The acute toxicity of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid (26.6% a.i.) to fish (zebrafish) was determined according to OECD Guideline 203 (1992) and EU Method C.1 (2008). An acute toxicity test under semi-static conditions with natural river water and daily renewal of the test media was conducted with the limit concentration of 146 µg/L of the test item, corresponding to a nominal dry content concentration of 38.8 µg/L. Duration of the test was 96 hours. Seven test organisms were exposed to the limit concentration and the control. Water quality parameters (temperature, pH-value and oxygen-saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits.
The limit concentration of the test item and the control were analytically verified via LC-MS/MS from freshly prepared media after 0 and 72 h and from corresponding 24 h aged test media after 24 and 96 h. The measured concentrations at the start of the exposure intervals after 0 and 72 hours were 94 and 100% of the nominal value. At the end the exposure intervals after 24 to 96 hours the measured test item concentrations were 41 and 39% of the nominal value.
Since the test is an UVCB substance all effect values given are based on the nominal concentration of the test item and its dry content. All validity criteria of the test guideline were met.
The LC0 was >/=146 µg/L in terms of test material, corresponding to >/=38.8 µg a.i./L.
The 96 h LC50 of the source substance DOPA-Glycinate in rainbow trout (Oncorhynchus mykiss) was 207.4 µg a.i./L (CL: 157.5–273.2 µg a.i./L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
