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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13 Dec 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Version / remarks:
1986
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-[[9,10-dihydro-9,10-dioxo-4-[(p-tolyl)amino]-1-anthryl]aminopropyl]trimethylammonium methyl sulphate
EC Number:
276-896-4
EC Name:
3-[[9,10-dihydro-9,10-dioxo-4-[(p-tolyl)amino]-1-anthryl]aminopropyl]trimethylammonium methyl sulphate
Cas Number:
72828-93-4
Molecular formula:
C27H30N3O2.CH3O4S C28H33N3O6S
IUPAC Name:
trimethyl[3-({4-[(4-methylphenyl)amino]-9,10-dioxo-9,10-dihydroanthracen-1-yl}amino)propyl]azanium methyl sulfate
Test material form:
solid
Details on test material:
Basic Blue 45 MeSO4

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Direct weighing into stock solution (1000 mg/L)
Dilution with test medium and addition of inoculum

Test organisms

Test organisms (species):
activated sludge, industrial
Details on inoculum:
Laboratory wastewater treatment plant
6.0 g dry particulate mater /L in stock solution

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Nominal and measured concentrations:
10, 18, 32, 56, 100 mg/L
Reference substance (positive control):
yes
Remarks:
dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
4.71 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
60.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
Respiration inhibition (compared to negative control): 24.5% (10 mg/L), 24.5% (18 mg/L), 32.1% (56 mg/L), 69.8% (100 mg/L)
Results with reference substance (positive control):
77% inhibition at 5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The substance caused significant respiration inhibition to activated sludge. The EC10 was determined at ca. 4.71 mg/L, the EC50 at ca. 60.5 mg/L.