4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with cyclohex-1,2-ylenediamine

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Samples were collected of the Stock Solution (WAF) and of each treatment at the beginning and end of exposure for analysis.

Vehicle:

no

Details on test solutions:

Algae medium was prepared in accordance with OECD 201. Specifically, the AAP
medium specified in Annex 3 from that method was used. Algae medium was prepared
using deionized water (DI). Each of the stock solutions was prepared in DI (Table 2).
One ml of each stock was added to a flask and diluted to one liter with DI water. This
water was mixed on a magnetic stir plate and filtered through a 0.2 micron filter for
sanitization. This medium was used for all culturing and testing of algae for this study.

Component Concentration in Prepared Medium (mg/L)
NaHCO3 15.0
NaNO3 25.5
MgCl2-6(H2O) 12.16
CaCl-2(H2O) 4.41
MgSO4-7H2O 14.6
K2HPO4 1.044
FeCl3-6(H2O) 0.160
Na2EDTA-2(H2O) 0.300
H3BO3 0.186
MnCl2-4(H2O) 0.415
ZnCl2 0.00327
CoCl2-6(H2O) 0.00143
Na2MoO4-(H2O) 0.00726
CuCl2-2(H2O) 0.000012

Test organisms (species):

other: Raphidocelis subcapitata

Details on test organisms:

An algal starter culture was purchased from Aquatic Biosystems, Inc. (Fort Collins,
Colorado) prior to testing. BMI cultures were inoculated at 104 cells/ml and allowed to
incubate for 72 hours. Cell counts were performed to ensure logarithmic growth. Testing
was performed using these cultures.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Nominal and measured concentrations:

Summary of Measured Concentrations
Nominal Concentration mg/L 0h mg/L 72h mg/L Concentration used for Calculations mg/L
0 BDL BDL 0
6.25 BDL BDL 0.03
12.5 BDL BDL 0.06
25 BDL BDL 0.12
50 BDL BDL 0.24
100 BDL BDL 0.48
Stock Solution 1 g/L (WAF) 4.8 4.8

Details on test conditions:

Test Requirement Condition
1. Test type: Static
2. Renewal of test solutions: Not Renewed
3. Temperature (°C): 22 + 2°C
4. Light quality: Full Spectrum
5. Light intensity: 60 - 100 μE/(m-2 s-1)
6. Photoperiod: Continuous
7. Size of test vessel: 250 ml
8. Volume of test solution: 100 ml
9. Cell Density at test initiation: 104 cells/ml
11. Number of replicates per concentration: 3
14. Aeration: None
15. Medium: See section 3.2
16. Test duration: 72 hours
17. Test concentrations: Five product concentrations and a control
18. Dilution series: 0.1 dilution series for range-finding test
0.5 dilution series for definitive test
19. Endpoint: Growth Inhibition
20. Sampling and sample: Treatments were prepared from WAF
21. Test acceptability criterion: Biomass of control cultures increase by a factor of at
least 16. Section – by – section specific growth rate
coefficient of variation not more than 35%.
Coefficient of variation during the whole test period
not greater than 7% in the controls.

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

not determinable

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.48 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The results of the range-finding study suggested that the endpoint would fall between 10 and 100 mg/L (Nominal WAF). Therefore, nominal treatments for the definitive test were 0, 6.25, 12.5 25, 50 and 100 mg/L WAF.

Treatment (mg/L nominal WAF) 72h Cell Count (Count X 104) Percent Inhibition
0 126 NA
.1 134 0
1 128 0
10 102 19
100 9 93
1000 0 100

Table presents a summary of the results from the toxicity test.

Measured Concentration (mg/L) 72h Cell Count (Average) Specific Growth Rate Percent Inhibition
0 129 0.067 NA
0.03 137 0.069 0
0.06 144 0.069 0
0.12 132 0.068 0
0.24 132 0.069 0
0.48 67 0.057 15.0

Validity criteria fulfilled:

yes

Conclusions:

Effect of 4,4’-Isopropylidenediphenyl, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with cyclohex-1,2-ylenediamine on the growth rate of Freshwater Alga was examined using OECD 201: 2006 test method. .The EC10 for specific growth rate was 0.40 mg/L based on measured concentrations. The EC50
for specific growth rate was > 0.48 mg/L. Based on the range-finding test, the EC50 is between
0.48 and 4.8 mg/L.

Executive summary:

Effect of 4,4’-Isopropylidenediphenyl, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with cyclohex-1,2-ylenediamine on the growth rate of  Freshwater Alga was examined using OECD 201: 2006 test method. .The EC10 for specific growth rate was 0.40 mg/L based on measured concentrations. The EC50

for specific growth rate was > 0.48 mg/L. Based on the range-finding test, the EC50 is between

0.48 and 4.8 mg/L.

Description of key information

Effect of 4,4’-Isopropylidenediphenyl, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with cyclohex-1,2-ylenediamine on the growth rate of  Freshwater Alga was examined using OECD 201: 2006 test method. .The EC10 for specific growth rate was 0.40 mg/L based on measured concentrations. The EC50 for specific growth rate was > 0.48 mg/L. Based on the range-finding test, the EC50 is between 0.48 and 4.8 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:

0.46 mg/L

EC10 or NOEC for freshwater algae:

0.4 mg/L