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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48-Hour EC50 for the test item to Daphnia magna based on nominal test concentrations was greater than 100 mg/l.  The No Observed Effect Concentration was 100 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

Introduction.

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

Methods.

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at a concentration of 100 mg/l for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours.

Results.

The 48-Hour EC50for the test item to Daphnia magna based on nominal test concentrations was greater than 100 mg/l. The No Observed Effect Concentration was 100 mg/l.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 90% to 105% of nominal value and so the results are based on nominal test concentrations only.