Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-479-2 | CAS number: 65122-08-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the structually and functionally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: refer below principle
- Principles of method if other than guideline:
- WoE study was prepared for toxicity of test chemical by considering WoE 2, 3 and 4
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Remarks:
- 2
- Details on test solutions:
- 2: The test substance was soluble in Adams media. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 0.125 mg/L,0.25 mg/L,0.5 mg/L,1 mg/L, and 2 mg/L, respectively. Test water:ADaM’s media is used, prepared in MiliQ water.
3: The stock solution 20 mg/L was prepared by dissolving dark violet iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 2: - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
3: TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding - Test type:
- other: 2: semi-static; 3: static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- 2: 145.5 mg of CaCO3
- Test temperature:
- 2: 20±2° C; 3: 20±1°C
- pH:
- 2: 7.1; 3: Test at 0.100 mg/l: 8.1 (changed to 7.9 during test)
Control: 7.7 (Changed to 7.9 during test) - Dissolved oxygen:
- 3: higher than 8.7 mg/L at the end of test
- Nominal and measured concentrations:
- 2: 0.5625 mg/L,1.125mg/L, 2.25mg/L, 4.5mg/L, 9mg/L; 3: 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l
- Details on test conditions:
- 2: TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
3: TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.
Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).
Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.
- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.
Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:
CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- 3: Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- 2
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.037 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 0.025-0.054 mg/l
- Remarks:
- 3
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 4
- Results with reference substance (positive control):
- 3: - Results with reference substance valid
- EC50: 0.76 mg/L (24 hours) - Reported statistics and error estimates:
- 3: EC50 was calculated using non linear regression by the software Prism 4.0
- Validity criteria fulfilled:
- yes
- Remarks:
- 2: 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized..2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Study was conducted to determine effecttive concentration of test chemical on aquatic invertebrates for 48 h. The EC50 value for aquatic invertebrates for test chemical was determined to be ranges from 0.037-0.5 mg/L
- Executive summary:
Data available for the structurally similar and closely related read across chemicals study has been reviewed to determine the short term toxicity of the test chemical on the growth of aquatic invertebrates. The studies are as mentioned below:
The first study of daphnia Immobilization Test was conducted in according to OECD Guideline 203 for reaction mass of test chemical . The test substance was soluble in Adams media. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 0.125 mg/L,0.25 mg/L,0.5 mg/L,1 mg/L, and 2 mg/L, respectively. Test water:ADaM’s media is used, prepared in MiliQ water and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for reaction mass of test chemical on test daphnia was determine to be 0.5 mg/L. Test consider to be valid as fulfills all the criteria as per standards, as not more that 10 percent of the daphnids were immobilized and the dissolved oxygen concentration at the end of the test was 3 mg/l in control and test vessels. Based on the EC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.
Second determination of the inhibition of the mobility of daphnids study was carried out with the substance according to OECD Guideline 202. The test substance was tested at the different nominal concentrations 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0. Potassium dichromate (K2Cr2O7) used as reference substance in study. The was carried out in 50 ml glass vessel of at temp of 20±1°C and 7.9 pH. The stock solution 20 mg/L was prepared by dissolving dark violet iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be 0.037 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 as per the CLP criteria.
The last short term toxicity to aquatic invertebrates study was performed in Daphnia magna for 48 hrs. After the experiment,the EC50 value for aquatic invertebrates for test chemical was determined to be 0.06 mg/l. Based on the value, test chemical was considered to be toxic in nature and can be classified in aquatic acute category 1 as per CLP regulation.
On the baisis of above observations, the EC50 value for aquatic invertebrates for test chemical was determined to be ranges from 0.037-0.5 mg/L. Based on the value, test chemical was considered to be toxic in nature and can be classified in aquatic acute category 1 as per CLP regulation.
Reference
OBSERVATION: |
||||
1. Immobility |
|
|||
Nominal |
Number |
of |
Immobility |
|
Concentration |
Daphnids |
0 h |
24 h |
48 h |
(mg/l) |
|
|
|
|
Control |
10 |
- |
- |
- |
0.125 |
10 |
- |
- |
- |
0.25 |
10 |
- |
- |
- |
0.5 |
10 |
- |
- |
5 |
1 |
10 |
- |
7 |
10 |
2 |
10 |
- |
10 |
10 |
9.2. pH and oxygen content: |
|
|
|
Nominal Concentration Number of |
pH(mg/L) |
|
|
(mg/l) |
daphnids |
0h |
48h |
Control |
10 |
7.3 |
7.7 |
0.125 |
10 |
7.5 |
7.6 |
0.25 |
10 |
7.5 |
7.7 |
0.5 |
10 |
7.6 |
7.8 |
1 |
10 |
7.6 |
7.8 |
2 |
10 |
7.6 |
7.9 |
Nominal Concentration Number of |
DO(mg/L) |
|
|
(mg/l) |
daphnids |
0h |
48h |
Control |
10 |
6.9 |
6.9 |
0.125 |
10 |
6.9 |
6.9 |
0.25 |
10 |
6.9 |
6.9 |
0.5 |
10 |
6.9 |
6.9 |
1 |
10 |
6.9 |
6.9 |
2 |
10 |
6.9 |
6.9 |
9.3. Test temperature |
|
|
|
Nominal Concentration Number of |
Temparature |
||
(mg/l) |
daphnids |
0h |
48h |
Control |
10 |
21.9 |
20.8 |
0.125 |
10 |
21.9 |
20.8 |
0.25 |
10 |
21.9 |
20.8 |
0.5 |
10 |
21.9 |
20.8 |
1 |
10 |
21.9 |
20.8 |
2 |
10 |
21.9 |
20.8 |
Description of key information
Study was conducted to determine effecttive concentration of test chemical on aquatic invertebrates for 48 h. The EC50 value for aquatic invertebrates for test chemical was determined to be ranges from 0.037-0.5 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.5 mg/L
Additional information
Data available for the structurally similar and closely related read across chemicals study has been reviewed to determine the short term toxicity of the test chemical on the growth of aquatic invertebrates. The studies are as mentioned below:
The first study of daphnia Immobilization Test was conducted in according to OECD Guideline 203 for reaction mass of test chemical . The test substance was soluble in Adams media. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 0.125 mg/L,0.25 mg/L,0.5 mg/L,1 mg/L, and 2 mg/L, respectively. Test water:ADaM’s media is used, prepared in MiliQ water and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for reaction mass of test chemical on test daphnia was determine to be 0.5 mg/L. Test consider to be valid as fulfills all the criteria as per standards, as not more that 10 percent of the daphnids were immobilized and the dissolved oxygen concentration at the end of the test was 3 mg/l in control and test vessels. Based on the EC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.
Second determination of the inhibition of the mobility of daphnids study was carried out with the substance according to OECD Guideline 202. The test substance was tested at the different nominal concentrations 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0. Potassium dichromate (K2Cr2O7) used as reference substance in study. The was carried out in 50 ml glass vessel of at temp of 20±1°C and 7.9 pH. The stock solution 20 mg/L was prepared by dissolving dark violet iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be 0.037 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 as per the CLP criteria.
The last short term toxicity to aquatic invertebrates study was performed in Daphnia magna for 48 hrs. After the experiment,the EC50 value for aquatic invertebrates for test chemical was determined to be 0.06 mg/l. Based on the value, test chemical was considered to be toxic in nature and can be classified in aquatic acute category 1 as per CLP regulation.
On the baisis of above observations, the EC50 value for aquatic invertebrates for test chemical was determined to be ranges from 0.037-0.5 mg/L. Based on the value, test chemical was considered to be toxic in nature and can be classified in aquatic acute category 1 as per CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.