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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, however no GLP, no analytical monitoring

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988
Reference Type:
other company data
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EU Directive 79/831/EEC, Annex V, part C
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
trimethylamine in aqueous solution 45 %
- Analytical purity: 99.9 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Preparation of test media:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test medium of the highest test concentration, 500 mg/l (nominal) was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. 10 ml of the respective dilutions were added to the test vessels.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Institute National de Recherche Chimique Appliquee, France. Cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h
- Weight at study initiation (mean and range, SD):
- Feeding during test: none

ACCLIMATION
- Type and amount of food: Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no data

Test conditions

Hardness:
2.70 ± 0.5 mmol/l
Test temperature:
19.85 °C
pH:
start: 8.22 - 10.49
48 h: 8.07 - 9.33
Dissolved oxygen:
start: 8.51 - 8.62
48 h: 7.94 - 8.12
Salinity:
no data
Nominal and measured concentrations:
Nominal concentrations (mg/l):
control, 15.62, 31.20, 62.50, 125.00, 250.00, 500.00
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water; pretreatment steps: 6 µm- and charcoal-filtration; H2SO4 was added to reduce alkalinity up to pH 4.3; distilled water was added to reduce water-hardness; water was aerated (oil-free air) unil saturated with oxygen; water was stored for at least 24 h for stabilization.
- Ca/mg ratio: 4:1


OTHER TEST CONDITIONS
- Photoperiod: 16:8 day-night period
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
139.95 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other: 95 % confidence limits: 123.1 - 154.6 mg/L

Any other information on results incl. tables

Cumulative immobilization of Daphnia after x hours [%]:

Nominal test concentration (mg/l)

3 h

6 h

24 h

48 h

Control

0

0

0

0

15.62

0

0

0

0

31.2

0

0

0

0

62.5

0

0

0

0

125

0

0

10

35

250

50

20

100

100

500

100

100

100

100

Remark in the report: toxicity at 250 mg/L and 500 mg/L may
be due to the high pH (up to 10.49) pH values after 0 and 48 hours Conc. [mg/L]       0 h       48 h 0                  8.22       8.07 15.62              8.75       8.11 31.2               9.13       8.17 62.5               9.5        8.26 125               9.86       8.51 250               10.19      8.97 500                10.49      9.33

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Results based on nominal concentrations: EC0: 62.5 mg/L, EC50: 139.95 mg/L, EC100: 250 mg/L
Executive summary:

Detailed information about the study design is given. Three different endpoints are given: EC0, EC50, and EC100. The nominal results are: 62.5 mg/L, 139.95 mg/L, and 250 mg/L, respectively. The basis for effect was mobility, the study time 48 hours.