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Ecotoxicological information

Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
Test duration too short, no information on the test substance given and documentation insufficient.
Principles of method if other than guideline:
Method: other: 10 animals/dose group; no further data
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Leuciscus idus melanotus
Test type:
not specified
Water media type:
freshwater
Total exposure duration:
48 h
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
ca. 10 440 mg/L

Result: LC50 = 9 ml/l

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Remarks:
Test procedure in accordance with national standard method with acceptable restrictions. REPORT NOT AVAILABLE.
Principles of method if other than guideline:
Method: other: DEV L15, DIN 38421, part 15 (1978)
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Leuciscus idus melanotus
Test type:
other: presumably static
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
11 600 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
>= 11 600 mg/L
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Pimephales promelas
Total exposure duration:
96 h
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
2 000 mg/L
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Study period:
1982-09-16 to 1982-09-18
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Qualifier:
according to
Guideline:
other: DIN 38 412 part 1
GLP compliance:
yes
Analytical monitoring:
no
Test organisms (species):
other: Idus melanotus
Water media type:
freshwater
Limit test:
no
Total exposure duration:
2 d
Duration:
2 d
Dose descriptor:
LC0
Effect conc.:
> 1 000 mg/L
Validity criteria fulfilled:
not specified
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Remarks:
only 48 h exposure
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
DIN 38412 Teil 15
Principles of method if other than guideline:
Method: other
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Leuciscus idus melanotus
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 900 mg/L
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
> 900 mg/L
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Remarks:
Test procedure in accordance with national standard method. But 2 fish died at 1000 mg/l, whereas at 1500 mg/l no animal was killed. STUDY SEEMS TO BE SAME AS Scholtz, N. 1987: Bestimmung der akuten wirkungen von SERVON XRK 60 gegenüber Fischen (nach DIN 38412 Teil 15). Hüls aktiengesellschaft DUV-Ps/BT Prüfinstitut für Biologie. Study has been performed already in 1987!. NO STUDY REPORT FROM THE YEAR 1997 AVAILABLE.
Principles of method if other than guideline:
Method: other: according to DEV DIN 38412 part 15, 1982
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Leuciscus idus melanotus
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
1 500 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1 500 mg/L
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No chemical analysis was performed.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
semi-static
Limit test:
no
Total exposure duration:
96 h
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 3 200 mg/L

After 96 h exposure at the highest concentration, the number of fish and their condition, visually assessed, were the same as those of the control fish. Therefore LC50 was higher than 3200 mg/l. The slope of the concentration-effect curve could not be calculated.

Endpoint:
short-term toxicity to fish
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Justification for type of information:
Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008).
Reason / purpose:
read-across source
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 3 200 mg/L
Conclusions:
After 96 h exposure at the highest concentration, the number of fish and their condition, visually assessed, were the same as those of the control fish. Therefore LC50 was higher than 3200 mg/l. The slope of the concentration-effect curve could not be calculated.

This information is used in a read-across approach in the assessment of the target substance.
Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008)
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Nominal and measured concentrations:
Nominal concentrations 1.000, 1.800, 3.200, 5.600 and 10.000 of QUAB 188 mg/L
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
2 094 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 128 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 6 544 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Reported statistics and error estimates:
The LC50 values and their confidence interval were calculated by means of a parametric model developed by Kooijman (1981).

NOEC 3200 mg (2094 mg/l), but in concentration 1800 mg/l (1178 mg/l) one fish died (the death is assumed to be accidental).

Validity criteria fulfilled:
yes
Conclusions:
A 96 h EC50 value of 4128 mg a.i./L has been determined for the effects of the test substance on mortality of Brachydanio rerio.

Description of key information

Eight short-term studies are available for fish. Seven studies were conducted with CHPTAC and one with its main degradation product 2, 3-Dihydroxy-N,N,N-trimethylpropan-1-aminium chloride. The determined effect concentrations are in a similar range and demonstrate that CHPTAC and its degradation product (diol) is not hazardous towards fish. The key value is determined as a 96-h LC50 of 4128 mg/L (nominal).

Key value for chemical safety assessment

LC50 for freshwater fish:
4 128 mg/L

Additional information

Overview of effect values:

Brachydanio rerio (new name: Danio rerio): 96-h LC50 = 4128 mg/L

Idus melanotus: 48-h LC0 = 1000 mg/L

Pimephales promelas: 96-h LC0 = 2000 mg/L

Leuciscus idus melanotus: 48-h LC0 = 11600 mg/L

48-h LC50 > 11600 mg/L

Leuciscus idus melanotus: 48-h LC0 = 1500 mg/L

48-h LC50 > 1500 mg/L

Leuciscus idus melanotus: 48-h LC50 = ca. 10440 mg/L

Leuciscus idus melanotus: 48-h LC0 > 900 mg/L

48-h LC50 > 900 mg/L

Effect concentration for the diol: Brachydanio rerio (new name: Danio rerio): 96-h LC50 > 3200 mg/L