N-[(triethoxysilyl)methyl]cyclohexylamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 01 - July 22, 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

adopted July 17, 1992

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

454-780-6

EC Name:

-

Cas Number:

27445-54-1

Molecular formula:

Hill formula: C12H27NO2Si CAS formula: C12H27NO2Si

IUPAC Name:

Cyclohexyl-((diethoxy-methyl-silanyl)-methyl)-amine

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Species: activated sludge, micro organisms from a domestic waste water
treatment plant
- Origin: supplied by the sewage plant GroB-Zimmem, Germany
- Conditioning: The activated sludge used for this study was washed by centrifugation and the supematant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged.
This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

25 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers or solvents were used, but the solutions were dispersed by stirring to achieve a homogeneous solution ofthe test item.
Test Item (flasks 1 and 2)
Inoculum Control (flasks 3 and 4)
Procedure Control (flask 5)
Abiotic Control (flask 6)
Toxicity Control (flask 7)
Incubation:
The closed test flasks were incubated in a climatic chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in the flasks. Evolved carbon dioxide was absorbed in an aqueous solution (45 %) ofpotassium hydroxide.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

29

Sampling time:

28 d

Remarks on result:

other: mean of two replicates

BOD5 / COD results

Results with reference substance:

The reference item Aniline was sufficiently degraded to 72 % after 14 days, and to 79 % after 28 days of incubation (based on ThOD N03).
A degradation of 93 % and 101 % were determined for 14 days and 28 days interval when calculation was done based on ThOD NH4.

Any other information on results incl. tables

The toxicity control attained 57% degradation after 14 d and 58 % after 28 days of incubation.

According to the test guidelines the test item can be assumed to be not inhibitory on the activated sludge micro organisms because degradation was> 25 % within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The percentage biodegradation of the test substance was 29 % at the end of the test under the conditions of the study, so the test substance cannot be considered to be readily biodegradable.