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EC number: 246-352-0 | CAS number: 24610-00-2
In this study the potential of Disperse Red 184 to induce chromosome aberrations was investigated in V 79 cells of the Chinese hamster lung in vitro. For each experiment two cell cultures were used.
Disperse Red 184 was suspended in DMSO. Evaluation of the solubility of that suspension in cell culture medium showed that 1000 µg/mL was the highest practicable concentration and produced precipitate. Accordingly, a preliminary toxicity study was carried out using a maximum concentration of 1000 µg/mL and a range of lower dose levels down to 10 µg/mL.
Following treatment in the absence of S9 metabolic activation, severe toxicity was observed at 500 µg/mL and above. Survival declined in a dose-related manner reaching 29.2 % of the solvent control value at the highest dose level, 1000 µg/mL.
In the presence of metabolic activation (S9-mix) there was only a slight indication of toxicity up to the limit of solubility.
Before treatment, the pH values and osmolality of the treatment media were determined. The addition of test compound solutions did not have any effect on these parameters.
Hence, the test compound was suspended in DMSO at the following concentrations:
20 h: 3.16#, 10.0#, 31.6, 100.0, 316.0 and 1000.0* µg/mL
28 h: 31.6#, 100.0#, 316.0 and 1000.0* µg/mL
20 h: 31.6#, 100.0, 316.0 and 1000.0 µg/mL
28 h: 316.0# and 1000.0 µg/mL
* not evaluated because of high toxicity
# not used because higher concentrations were evaluated
The highest concentrations produced no relevant lowering of the mitotic index in the presence of metabolic activation and a distinct lowering of the mitotic index in the absence of metabolic activation. Microscopic visible precipitation of the test compound was observed at 10 µg/mL and above in the absence of S9-mix and at 100 µg/mL and above in the presence of S9-mix.
After treatment with the test compound there was no relevant increase in the number of polyploid cells as compared with the solvent controls.
There was an enhancement of the aberration rates inclusive and exclusive gaps 20 h and 28 h after the start of the treatment with the concentration of 316 µg/mL (relative mitotic index 19.4% and 44%, respectively) without S9-mix. In addition, an increased number of cells with break events was found in these groups. In the presence of S9-mix no relevant reproducible enhancement of metaphases over the range of the solvent control was found with any of the concentrations used.
The sensitivity of the test system was demonstrated by the enhanced mutation frequency in the cell cultures treated with the positive control compounds.
As the increase in aberration rates were only observed at 316 µg/mL without metabolic activation and in the presence of cytotoxicity and visible test substance precipitation, the relevance and reliability of this result is questionable.
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