Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Method similar to guideline but no analytical, and test species is not the standard species
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
no chemical analysis; Goldorfen is not the standard test species but is among those often tested
GLP compliance:
no
Specific details on test material used for the study:

- Name of test material (as cited in study report): p-toluene sulfonic acid
- Substance type: organic
- Physical state: liquid
- Analytical purity: 65% active ingredient
- Purity test date: not indicated
- Lot/batch No.: not indicated
- Storage condition of test material: not indicated
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
diluted in water only
Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
TEST ORGANISM
- Common name: Goldorfen
- Source: Paul Eggers, 2345 Hohenwestedt
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 5.5 - 6.6 cm
- Weight at study initiation (mean and range, SD): 1.5-2.7 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: no indicted
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min
- Feeding frequency: not indicated
- Health during acclimation (any mortality observed): not indicated
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
114 mg CaCO3/L
Test temperature:
20 +/- 1 C
pH:
7.5 - 8.2 (except at 500 mg/L where it was 5.7)
Dissolved oxygen:
8.5 - 9.2 mg/L
Salinity:
N/A
Nominal and measured concentrations:

Nominal - 0, 10, 100 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquarium with 20L solution
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 20L
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Ca/mg ratio: 0.7
- Na/K ratio: 21
- Intervals of water quality measurement: 0, 2, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality daily; observations regularly

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5-10X
- Range finding study: not indicated
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
>325 mg/L based on active ingredient
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
yes
Conclusions:
Test substance was not toxic at 500 mg/L (325 mg/L active substance), the highest concentration tested up to 96hours.

Description of key information

Test substance was not toxic at 500 mg/L (325 mg/L active substance), the highest concentration tested up to 96hours.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
500 mg/L

Additional information