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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-02-13 to 1995-02-17
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Principles of method if other than guideline:
At defined concentrations of a substance arise after the test period different percentages of dead fish. The LC 0 and LC 100 values were determined directly, while the LC 50 value is determined by calculation or graphically from the concentration-response curve.
The test duration is 96 hours.
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Test concentrations: 5, 10, 20, 40 and 80 mg/L
- direct weighing
Vehicle:
no
Details on test solutions:
- Conditions: static test according to the Guideline
- Light-dark phase: 16 h light / 8h dark
- Test procedure: Test aquaria 300 x 135 x 200 mm; 5 L test medium, ventilated; static
- Tools: Magnetic
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Type: zebrafish (Danio rerio HAMILTON BUCHANAN)
- Origin: West Aquarium (Bad Lauterberg)
- Date of birth: 1994-09-11
- Ship Date: 1994-12-06
- Body length: 2.5 - 3.5 cm
- Storage under certain conditions: SOP 4001
- Mortality at storage: <2 %
- Medical treatment: no
- Water quality at storage: synthetic freshwater according to ISO
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
14.3 °dH
Test temperature:
Control: 21.3 - 21.8 °C
Test item: 21.4 - 21.7 °C
pH:
Control: 7.6 - 8.1
Test item: 7.7 - 8.1
Dissolved oxygen:
Control: 8.4 - 9.0 mg/L
Test item: 8.5 - 9.3 mg/L
Nominal and measured concentrations:
Nominal concentrations: 5, 10, 20, 40 and 80 mg/L
Details on test conditions:
The substance was weighed in water to produce the individual test concentrations stirred for 3 hours on a magnetic stirrer.
The result specification refers to the substance, calculated from the measured TOC values (1 mg/L TOC is equivalent 1.2 mg/L test substance).
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
42.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
21.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Geometric mean: LC0 / LC100 : LC50 = 21.4 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The arithmetic mean of LC0 / LC100 was calculated as LC50 = 21.4 mg/L after 96 hours esposure.
Executive summary:

The acute toxicity of dibenzylamine to Fish (Danio rerio) was determined in a 96 -hour static test according to EU method C.1 'Acute toxicity for Fish' (1992). Under the conditions of the present study, the 96 -h LC50 (arithmetic mean) was calculated as 21.4 mg/L.

Description of key information

The study of Bayer (1995) investigating the acute toxicity of dibenzylamine to fish according to EU Method C.1 was considered as the key study for endpoint coverage (klimisch score of 2). An EC50 of 21.4 mg/L (based on arithmetic mean concentration) was obtained after an exposure of 96 hours in a static test in freshwater.

Key value for chemical safety assessment

LC50 for freshwater fish:
21.4 mg/L

Additional information